Anti-Mouse CD4 (Clone 131922)

Product No.: C1439


Clone 131922 Target CD4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD4mut, L3T4, Ly-4, CD4 Antigen Isotype IgG2b Applications WB


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Antibody Details Product Details Reactive Species Mouse Host Species Rat Immunogen NS0-Derived Recombinant Mouse CD4 Extracellular Domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2828500 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Mouse CD4 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CD4 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Rat Anti-Mouse CD4 (Clone 131922) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.¹ PubMed CD4 NCBI Gene Bank ID 12504 UniProt.org Information on Uniprot.org References & Citations 1. Hendrickson, WA. et al. (1994) Structure 2: 59 View product citations for antibody PRODUCT_CODE on CiteAb Technical Protocols Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Rat

Immunogen

NS0-Derived Recombinant Mouse CD4 Extracellular Domain

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2828500

Applications and Recommended Usage?
Quality Tested by Leinco

Western Blotting: To detect Mouse CD4 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse CD4 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Rat Anti-Mouse CD4 (Clone 131922) recognizes an epitope on Mouse CD4. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

CD4 (cluster of differentiation 4) is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. CD4 interacts with class II molecules of the major histocompatibility complex (MHC) enhancing the signal for T-cell activation.¹

PubMed

CD4

NCBI Gene Bank ID

12504

UniProt.org

Information on Uniprot.org

References & Citations

1. Hendrickson, WA. et al. (1994) Structure 2: 59

View product citations for antibody PRODUCT_CODE on CiteAb

Certificate of Analysis

Request COA

Prod No.	Description
C1840	Anti-Mouse CD4 (Clone GK1.5) – PerCP
C211	Anti-Mouse CD4 -(Clone GK1.5) Purified
C214	Anti-Mouse CD4 (Clone GK1.5) – PE
C212	Anti-Mouse CD4 (Clone GK1.5) – Biotin
C213	Anti-Mouse CD4 (Clone GK1.5) – FITC
C338	Anti-Mouse CD4 (Clone GK1.5) – APC
C1640	Anti-Mouse CD4 (Clone GK1.5) – DyLight® 650
C1636	Anti-Mouse CD4 (Clone GK1.5) – DyLight® 488
C1333	Anti-Mouse CD4 Antibody (Clone GK1.5) – Purified in vivo GOLD™ Functional Grade
C1638	Anti-Mouse CD4 (Clone GK1.5) – DyLight® 594
C1637	Anti-Mouse CD4 (Clone GK1.5) – DyLight® 550
C2838	Anti-Mouse CD4 (Clone GK1.5) – Purified in vivo PLATINUM™ Functional Grade
C1439	Anti-Mouse CD4 (Clone 131922)
C1317	Recombinant Human CD4 (Soluble)

Formats Available

Prod No.	Description
C1439	Anti-Mouse CD4 (Clone 131922)

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Mouse CD4 (Clone 131922)