Anti-Mouse CD62L [Clone MEL-14] — Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD62L [Clone MEL-14] — Purified in vivo PLATINUM™ Functional Grade
Product No.: C6118
Clone MEL-14 Target CD62L Formats AvailableView All Product Type Monoclonal Antibody Alternate Names L-selectin, LECAM-1, Ly-22, LAM-1, MEL-14 Isotype Rat IgG2a κ Applications B , CyTOF® , Depletion , FA , FC , IHC FF , in vivo , IP , WB |
Data
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen C3H/eb mouse B lymphoma 38C-13 Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829778 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this MEL-14 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this MEL-14 antibody for use in western blotting is 1-10 μg/ml. Additional Applications Reported In Literature ? CyTOF® IHC FF IP B Additional Reported Applications For Relevant Conjugates ? IHC (Paraffin) For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Rat Anti-Mouse CD62L (Clone MEL-14) recognizes an epitope on Mouse CD62L. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background CD62L is a 74-95 kD glycoprotein is a member of the selectin family and is expressed on the majority of B and naïve T cells, a subset of memory T cells, monocytes, granulocytes, most thymocytes, and a subset of NK cells. Furthermore, CD62L is a cell adhesion molecule that binds to many glycoprotein ligands including CD34, GlyCAM-1, and PSGL-1. Clone MEL-14 is reported as a Nutralization antibody. Antigen Distribution Subsets of B and T cells, monocytes, granulocytes, subset of NK cells Ligand/Receptor CD34, GlyCAM-1, MAdCAM-1 Function Lymphocyte homing to HEV, rolling on activated endothelium PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Costimulatory Molecules . Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone MEL-14 is a rat monoclonal antibody targeting mouse CD62L (L-selectin) and is used in vivo mouse studies primarily for two applications: functional blockade of lymphocyte homing to lymph nodes and as a surface marker for flow cytometric identification of lymphocyte subpopulations. Key uses in in vivo mouse studies:
Important considerations:
Summary Table: MEL-14 Uses in In Vivo Mouse Experiments
In summary, MEL-14 is chiefly used in vivo to block lymphocyte trafficking to lymph nodes, allowing researchers to dissect the role of cellular localization in immune responses, as well as to characterize lymphocyte phenotypes ex vivo after in vivo manipulations. The correct storage temperature for the sterile packaged clone MEL-14 antibody is 2–8°C (typically a standard refrigerator temperature), stored undiluted and protected from prolonged exposure to light. Do not freeze the antibody. Additional details:
Commonly used antibodies or proteins frequently reported in the literature alongside MEL-14 (anti-mouse CD62L/L-selectin) include markers of T and B cell subsets and activation, as well as additional adhesion molecules and trafficking markers. Frequently used antibodies/proteins with MEL-14:
Context and experimental rationale:
Experimental protocols and applications:
Alternative nomenclature: In the literature, CD62L may also be referenced as L-selectin, LECAM-1, Ly-22, LAM-1, or lymphocyte homing receptor. In sum, MEL-14 is most often used together with markers for lymphocyte subset identification, activation (e.g., CD44), and other trafficking-related proteins (e.g., integrins, selectins, chemokine receptors) when studying lymphocyte biology and homing in mice. The MEL-14 clone has generated significant findings in scientific literature, particularly regarding its role in understanding lymphocyte trafficking and selectin function. This rat anti-mouse CD62L monoclonal antibody has been instrumental in advancing our knowledge of cell adhesion mechanisms and immune cell migration. Recognition and Binding PropertiesMEL-14 recognizes mouse L-selectin (CD62L), an adhesion molecule expressed on most T and B lymphocytes, neutrophils, monocytes, and eosinophils. The antibody specifically binds to an epitope within the lectin domain of the murine peripheral lymph node homing receptor (pln HR). Through detailed epitope mapping studies, researchers determined that the MEL-14 recognition site is located within the NH2-terminal 53 amino acids of the lectin domain. Functional Blocking CapabilitiesOne of the most significant findings is MEL-14's ability to completely and specifically block lymphocyte-endothelial interactions. Pre-incubation of lymphocytes with MEL-14 completely blocks binding of lymphocytes to high endothelial venules (HEV) in vitro and prevents the migration of lymphocytes to lymph nodes in vivo. This blocking activity demonstrates that MEL-14 interferes with the same cellular interactions that are inhibited by PPME, a yeast cell wall polyphosphomannan carbohydrate. Structural Insights and Domain DependenciesResearch using MEL-14 revealed important structural requirements for proper epitope recognition. While the antibody recognized truncated homing receptor constructs containing both the lectin and EGF domains, antibody recognition was lost when the lectin domain alone was expressed. This finding suggests that the MEL-14 epitope's conformation may be dependent upon the presence of the EGF domain, highlighting the importance of proper protein folding and domain interactions for antibody recognition. Mechanistic Understanding of Lymphocyte TraffickingThe functional blocking capabilities of MEL-14 have provided crucial evidence supporting the hypothesis that the peripheral lymph node homing receptor's lectin domain is directly involved in binding lymphocytes to carbohydrate ligands on peripheral postcapillary venule endothelium. This finding has been fundamental to understanding the molecular mechanisms underlying lymphocyte homing and trafficking between blood circulation and lymphoid tissues. Technical Applications and Research ImpactMEL-14 has proven valuable across multiple research applications, including flow cytometry, immunohistochemistry, and functional assays. Its ability to efficiently block lymphocyte-endothelial interactions has made it an essential tool for studying immune cell migration patterns and understanding the role of selectins in inflammatory processes. The antibody's specificity and blocking function have established it as a standard reagent for investigating L-selectin-mediated cellular interactions in both basic research and applied studies of immune system function. References & CitationsTechnical ProtocolsCertificate of Analysis |
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