Anti-Mouse Vγ2 TCR – Purified in vivo GOLD™ Functional Grade

Anti-Mouse Vγ2 TCR – Purified in vivo GOLD™ Functional Grade

Product No.: T353

[product_table name="All Top" skus="T353"]

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Clone
UC3-10A6
Target
Vγ 2 T-Cell Receptor
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Vγ2 T-cell receptor, T cell receptor gamma 2
Isotype
IgG
Applications
Depletion
,
FC
,
in vivo
,
IP

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Armenian Hamster
Recommended Dilution Buffer
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 µg per 1.0 X 106 cells in a 100 µl total staining volume, followed by Goat-anti-Hamster IgG (PN:A237). Each lot of this antibody is quality control tested.
Additional Applications Reported In Literature ?
Depletion:The UC3-10A6 antibody has been shown to deplete γδ T cells when administered in vivo.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
This antibody reacts with Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes.
Background
The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules.
Antigen Distribution
Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of γδ TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung.
Ligand/Receptor
Antigens
Function
Studies suggest that these cells recognize bacterial ligands and some tumor cells.
Research Area
Adaptive Immunity
.
Immunology

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

Clone UC3-10A6 is most commonly used in vivo in mice to specifically deplete γδ T cells expressing the Vγ2 T cell receptor.

Essential context and supporting details:

  • The UC3-10A6 antibody targets the mouse TCR Vγ2 on γδ T cells and, when administered in vivo, is routinely used to selectively deplete this γδ T cell population in experimental mice.
  • This antibody is applied in settings where researchers want to study the biological roles of Vγ2+ γδ T cells, such as their contribution to immune responses in infection, autoimmunity, tumor immunity, and tissue homeostasis.
  • Additional reported applications include:
    • Functional assays investigating γδ T cell biological pathways.
    • Potential use in bioanalytical pharmacokinetic (PK) and anti-drug antibody (ADA) assays.
    • Occasionally, for in vivo neutralization (functional blockade rather than depletion), but the primary in vivo role is depletion.

Key additional information:

  • The antibody is a recombinant hamster IgG and is typically produced at low endotoxin levels suitable for mouse injection.
  • Vγ2+ γδ T cells are a significant subset in mouse tissues such as the thymus, peripheral lymphoid organs, lung, gut, and skin, playing diverse but not fully elucidated roles in immune regulation.
  • Depletion is achieved because the antibody induces clearance (or functional inactivation) of the targeted γδ T cells, allowing for mechanistic studies by comparing depleted versus non-depleted mice in various disease or immunological models.

In summary, UC3-10A6 is a research tool primarily used to deplete Vγ2+ γδ T cells in vivo in mice for the purpose of dissecting their function in health and disease.

The antibody UC3-10A6 is frequently used to identify or deplete mouse Vγ2 TCR-expressing γδ T cells. In the literature, it is commonly used in combination with other antibodies and proteins that help characterize T cell populations or increase experimental specificity.

Commonly used antibodies or proteins with UC3-10A6 include:

  • Anti-CD3 antibody: Used to identify all T cells by targeting the CD3 molecule, which is a component of the T cell receptor complex.
  • Anti-γδ TCR antibody: Broadly labels all γδ T cells, allowing for discrimination between γδ TCR-positive and other cells, whereas UC3-10A6 specifically labels the Vγ2 subset.
  • Anti-CD4 and Anti-CD8 antibodies: Used for further subtyping of T cells into helper and cytotoxic T cells, respectively. These are standard lineage markers in T cell immunophenotyping.
  • Isotype control antibodies: For ensuring specificity of staining, Armenian Hamster IgG isotype controls are typically selected when working with UC3-10A6.
  • Secondary antibodies (e.g., Goat-anti-Hamster IgG): Employed for signal amplification, especially when using unconjugated UC3-10A6.

Applications where these antibodies are used together:

  • Flow cytometry: UC3-10A6 is often used in multicolor panels along with other markers such as CD3, CD4, CD8, and pan-γδ TCR.
  • In vivo depletion: UC3-10A6 can be used to selectively deplete Vγ2 γδ T cells, sometimes in combination with other depleting antibodies for different cell types.
  • Functional studies: Co-staining with activation or cytokine markers (e.g., anti-IFN-γ) is also common when assessing activation following stimulation.

Relevant literature examples:

  • Mamedov et al. used anti-CD3, pan-γδ TCR, and anti-IFN-γ antibodies together with UC3-10A6 for flow cytometric characterization of γδ T cells and their cytokine production in mice.

Summary Table: Common Antibodies Used with UC3-10A6

Marker/AntibodyPurpose
CD3Pan-T cell marker
γδ TCR (pan)Detects all γδ T cells
CD4, CD8T cell subset characterization
IFN-γ, TNF-α, etc.Cytokine/functional assessment
Isotype controlStaining specificity control
Goat-anti-Hamster IgGSecondary for unconjugated UC3-10A6

These antibody combinations enable precise identification, sorting, and functional analysis of γδ T cell subsets in mouse immunology studies.

The monoclonal antibody clone UC3-10A6 is widely used in scientific literature for the identification and manipulation of mouse γδ T cells expressing the Vγ2 T cell receptor (also known as Vγ4 under different nomenclature systems). The key findings from citations and applications of this clone are:

  • Specificity & Nomenclature: UC3-10A6 specifically recognizes the Vγ2 TCR segment (designated Vγ2 by Garman nomenclature, also known as Vγ4 in Tonegawa nomenclature). This precision makes it a standard tool for targeting this subset of γδ T cells in mouse models.

  • Functional Use in Research:

    • The antibody is extensively used for flow cytometry to stain, identify, and sort Vγ2+ (Vγ4+) γδ T cells in various mouse tissues, evaluating their distribution and function.
    • In vivo depletion: UC3-10A6 has demonstrated efficacy in depleting Vγ2+ γδ T cells in vivo, thereby allowing functional studies into their roles by comparing outcomes before and after depletion.
  • Biological Insights from UC3-10A6 Studies:

    • Immunological surveillance: Vγ2 TCR γδ T cells constitute a major portion of the total γδ T cell population in murine fetal and adult lymphoid tissues and are implicated in immune surveillance, particularly for self-antigens induced under stress.
    • Genetic Independence: The frequency of Vγ2+ cells varies widely among mouse strains (12% to 54%) but does not correlate with MHC haplotype, suggesting an MHC-independent mechanism for their expansion or regulation.
    • Disease relevance: When used for cell depletion or identification, studies have uncovered roles in autoimmunity (e.g., arthritis), infection response, and potentially tumor immunology by selectively impacting Vγ2+ (Vγ4+) cell populations.
  • Technical Parameters:

    • The antibody is available conjugated to various fluorophores, suitable for multiparameter flow cytometry.
    • It is an Armenian hamster IgG isotype.
    • Optimal use requires titration specific to the experimental context.
  • Cited Research Applications:

    • UC3-10A6 has been cited in at least 12 publications for applications including flow cytometry, in vivo cell depletion, and immune cell characterization.
    • Its use has been foundational in characterizing the immunological function of Vγ2+ γδ T cells across diverse immunological settings, including inflammation, infection, and cancer.

In summary: UC3-10A6 is a validated and widely cited monoclonal antibody for the detection, quantification, and functional manipulation of mouse Vγ2 (Vγ4) γδ T cells, providing critical insights into their biology—especially in immune surveillance, disease models, and the functional consequences of their selective depletion.

Dosing regimens for clone UC3-10A6—an anti-mouse Vγ2 TCR monoclonal antibody—used for in vivo γδ T cell depletion in mouse models are not standardized and vary according to experimental design, mouse strain, and research purpose. Most sources recommend that investigators determine the optimal dosing based on their specific application, but typical regimens are inferred from related antibody depletion studies.

Essential context and supporting details:

  • Common in vivo application: UC3-10A6 is mainly used to deplete Vγ2-expressing γδ T cells in mouse models, targeting immune regulation or disease progression.

  • Suggested dosing range: Specific published regimens for UC3-10A6 are rare; however, dosing for similar hamster IgG antibodies used for T-cell depletion in mice generally falls in the range of 100–250 μg per mouse per injection, administered intraperitoneally (i.p.), often every 3–7 days.

  • Strain variability: Vγ2+ cell frequencies differ significantly across mouse strains (12% to 54% of the γδ T cell population), which may influence dosing requirements for effective depletion. Therefore, the optimal dose might need adjustment based on the strain used.

  • Protocol flexibility: Suppliers and manufacturers (Leinco, Bio X Cell, BioLegend) explicitly recommend that each investigator optimize their protocol through pilot studies: "Each investigator should determine their own optimal working dilution for specific applications," and to consult lot-specific datasheets, as dosing information can change.

  • Exemplar for in vitro use: For flow cytometry, typical concentrations are much lower (≤0.25–1.0 µg per 1×10⁶ cells in 100 µl volume), not directly relevant to in vivo depletion.

  • Experimental precedent: Some published protocols for other γδ T cell depleting antibodies suggest dosing before key interventions (e.g., vaccination or challenge), such as giving the antibody one day prior to treatment and again just before disease challenge. The frequency and total dose should be adapted to study design.

Additional relevant information:

  • Isotype: Armenian hamster IgG, κ, unconjugated.
  • Administration route: Most anti-TCR antibodies for depletion are given intraperitoneally, but intravenous injection may also be used depending on experimental needs.
  • Storage: Antibody solution should be stored at 4°C, not frozen.

In summary, there is no single standardized regimen for UC3-10A6 in mouse models, and effective dosing must be empirically established for each project, typically falling within the 100–250 μg per mouse, given intraperitoneally every 3–7 days, with adjustments required for mouse strain and experimental goal.

References & Citations

1. Hedrick, SM. et al. (1990) Nature. 343:714
Depletion
Flow Cytometry
in vivo Protocol
Immunoprecipitation Protocol

Certificate of Analysis

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.