Anti-Mouse Vγ2 TCR – Purified in vivo GOLD™ Functional Grade
Anti-Mouse Vγ2 TCR – Purified in vivo GOLD™ Functional Grade
Product No.: T353
Clone UC3-10A6 Target Vγ 2 T-Cell Receptor Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Vγ2 T-cell receptor, T cell receptor gamma 2 Isotype IgG Applications Depletion , FC , in vivo , IP |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Armenian Hamster Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2894354 Applications and Recommended Usage? Quality Tested by Leinco FC: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 µg per 1.0 X 106 cells in a 100 µl total staining volume, followed by Goat-anti-Hamster IgG (PN:A237). Each lot of this antibody is quality control tested. Additional Applications Reported In Literature ? Depletion:The UC3-10A6 antibody has been shown to deplete γδ T cells when administered in vivo. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody reacts with Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes. Background The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules. Antigen Distribution Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of γδ TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung. Ligand/Receptor Antigens Function Studies suggest that these cells recognize bacterial ligands and some tumor cells. PubMed Research Area Adaptive Immunity . Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone UC3-10A6 is most commonly used in vivo in mice to specifically deplete γδ T cells expressing the Vγ2 T cell receptor. Essential context and supporting details:
Key additional information:
In summary, UC3-10A6 is a research tool primarily used to deplete Vγ2+ γδ T cells in vivo in mice for the purpose of dissecting their function in health and disease. The antibody UC3-10A6 is frequently used to identify or deplete mouse Vγ2 TCR-expressing γδ T cells. In the literature, it is commonly used in combination with other antibodies and proteins that help characterize T cell populations or increase experimental specificity. Commonly used antibodies or proteins with UC3-10A6 include:
Applications where these antibodies are used together:
Relevant literature examples:
Summary Table: Common Antibodies Used with UC3-10A6
These antibody combinations enable precise identification, sorting, and functional analysis of γδ T cell subsets in mouse immunology studies. The monoclonal antibody clone UC3-10A6 is widely used in scientific literature for the identification and manipulation of mouse γδ T cells expressing the Vγ2 T cell receptor (also known as Vγ4 under different nomenclature systems). The key findings from citations and applications of this clone are:
In summary: UC3-10A6 is a validated and widely cited monoclonal antibody for the detection, quantification, and functional manipulation of mouse Vγ2 (Vγ4) γδ T cells, providing critical insights into their biology—especially in immune surveillance, disease models, and the functional consequences of their selective depletion. Dosing regimens for clone UC3-10A6—an anti-mouse Vγ2 TCR monoclonal antibody—used for in vivo γδ T cell depletion in mouse models are not standardized and vary according to experimental design, mouse strain, and research purpose. Most sources recommend that investigators determine the optimal dosing based on their specific application, but typical regimens are inferred from related antibody depletion studies. Essential context and supporting details:
Additional relevant information:
In summary, there is no single standardized regimen for UC3-10A6 in mouse models, and effective dosing must be empirically established for each project, typically falling within the 100–250 μg per mouse, given intraperitoneally every 3–7 days, with adjustments required for mouse strain and experimental goal. References & Citations1. Hedrick, SM. et al. (1990) Nature. 343:714 Technical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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T554 | |
T212 | |
T219 | |
T353 | |
T211 | |
T213 | |
T594 | |
T704 | |
T210 |
