Anti-Mouse Vγ2 TCR – Purified in vivo PLATINUM™ Functional Grade

The clone UC3-10A6, which targets the mouse TCR Vγ2, is commonly used in in vivo studies in mice for several applications:

1. Depletion of γδ T Cells: This antibody has been shown to effectively deplete γδ T cells when administered in vivo. This is particularly useful in research where the role of γδ T cells needs to be studied or when their depletion is required for experimental purposes.

2. Flow Cytometry: Clone UC3-10A6 is used in flow cytometry for the identification and enumeration of cells expressing TCR Vγ2. This method helps in analyzing the phenotype and distribution of γδ T cells in vivo.

3. Immune System Studies: The antibody aids in studying the functions and interactions of γδ T cells within the immune system, especially in the context of bacterial infections or cancer research.

4. Functional Assays: It is also used in functional assays to study biological pathways affected by TCR Vγ2, although this is more of a general application for similar antibodies.

The antibody UC3-10A6 is commonly used to detect the mouse TCR Vγ2 (gamma delta T cell receptor V gamma 2), mainly in studies of mouse γδ T cells. In the literature, several other antibodies and proteins are frequently used alongside UC3-10A6 for multiparametric analyses, including characterization of T cell populations and functional studies.

Commonly used antibodies/proteins with UC3-10A6 include:

• Pan-γδ TCR antibodies: These broadly detect all γδ T cells, often used to distinguish total γδ T cells from Vγ2+ γδ subsets detected by UC3-10A6.
• Other Vγ or Vδ segment-specific antibodies: Antibodies specific for other Vγ chains (such as Vγ1, Vγ4—note the nomenclature overlap with Vγ2) and Vδ chains help differentiate subpopulations within γδ T cells in flow cytometry panels or functional assays.
• CD3 antibody: Commonly used to mark all T cells or exclude non-T cell populations in immunophenotyping.
• CD4 and CD8 antibodies: Used to distinguish αβ T cell subsets and sometimes gate out these populations to focus analyses on γδ T cells.
• Macrophage and myeloid cell markers: Depending on the study focus, macrophage markers (such as F4/80 or CD11b) may be used for characterization of co-cultures or tissue infiltrates.
• Activation or differentiation markers: Antibodies for markers like CD69, CD25, CD44, and others are often used to study activation status of Vγ2+ γδ T cells.
• Cytokine detection antibodies: IFN-γ, IL-17, and TNF-α intracellular staining antibodies are frequently used to characterize the functional states of γδ T cells upon stimulation.

Typical example of a flow cytometry panel using UC3-10A6:

• Anti-mouse Vγ2 (UC3-10A6, with various fluorophore conjugates—e.g., PE, APC, FITC)
• Anti-TCRγδ (pan, clone GL3 or similar)
• Anti-CD3 (e.g., clone 17A2)
• Anti-CD4 and Anti-CD8
• Anti-CD45 (leukocyte marker)
• Fixable viability dye

Conjugate choices:
UC3-10A6 is available in several fluorophore conjugates (PE, APC, FITC, PerCP/Cy5.5, V500, DyLight488, TotalSeq barcoded formats) making it highly adaptable to complex multiparameter panels.

Isotype controls and dilution buffers:
Appropriate Armenian hamster IgG isotype controls and suitable dilution buffers are recommended for experimental controls and optimization.

Summary:
Researchers most frequently use UC3-10A6 together with antibodies for pan-γδ TCR, other Vγ/Vδ chains, CD3, CD4, CD8, and functional markers. This enables comprehensive analysis of γδ T cell biology in mouse models through multiparametric flow cytometry and functional assays.

The key findings from clone UC3-10A6 citations in scientific literature primarily revolve around its use as a monoclonal antibody to identify and study mouse Vγ2 T cells. Here are some key points:

1. Specificity and Application: Clone UC3-10A6 is specific for the Vγ2 segment of the T cell receptor (TCR) on γδ T cells in mice. It has been widely used in flow cytometric analysis to study these cells in various lymphoid tissues.

2. Use in Immunological Studies: This antibody is crucial for investigating the role of γδ T cells, particularly those expressing the Vγ2 chain, in immune responses. It aids in understanding their contribution to health and disease, such as in models of infection or autoimmune diseases.

3. In Vivo Depletion: UC3-10A6 can be used to deplete Vγ2+ γδ T cells in mice, which helps researchers study the specific functions of these cells in vivo. This method is essential for understanding their implications in disease models.

4. Nomenclature Consistency: The clone UC3-10A6 is said to react with the Vγ2 segment as designated by the Garman nomenclature, which is equivalent to Vγ4 under the Heilig & Tonegawa nomenclature system. This consistency is important for ensuring accurate identification across different nomenclature systems.

Scientific citations involving this clone often highlight its utility in characterizing γδ T cells and their roles in immune regulation and disease. However, specific outcomes from these studies can vary based on the context of the research, such as the type of disease model or the specific immune response being studied.

The dosing regimens of clone UC3-10A6, an anti-mouse Vγ2 TCR antibody, are not standardized and vary significantly depending on the experimental context and objectives of different mouse model studies.

General Dosing Characteristics

Clone UC3-10A6 is an Armenian hamster IgG monoclonal antibody that targets the delta chain of the mouse Vγ2 TCR (also known as Vγ4 under alternative nomenclature). The antibody has been reported to deplete γδ T cells when administered in vivo, though the specific mechanisms and dosing requirements differ across applications.

Documented Dosing Examples

In studies involving γδ T cell depletion, researchers have used 200 μg of the UC3-10A6 antibody administered to mice one day prior to each vaccination and just before challenge. This dosing approach was part of a regimen designed to deplete Vγ4+ γδ T cells in sterile immunity models.

For comparative purposes, other anti-TCR-δ antibodies used in similar applications have employed different dosing strategies. Some studies have used anti-TCR-δ antibody mixtures (combining GL3 and 403A10 clones in a 1:1 ratio) at 200 μg doses, while others have utilized increasing doses delivered via aerosolized administration.

Factors Influencing Dosing Variation

The lack of standardization in UC3-10A6 dosing regimens stems from several factors inherent to different experimental designs. The route of administration (intraperitoneal injection being most common for in vivo antibodies), the frequency of dosing (single dose versus repeated administrations), and the specific research objectives (complete depletion versus functional modulation) all contribute to dosing variability.

Additionally, the mouse strain and the distribution of Vγ2+ γδ T cells in different tissues can influence optimal dosing. These cells represent a large proportion of γδ T cells in late fetal and adult thymus, peripheral lymphoid tissues, lung, intestinal epithelium, and epidermis, meaning tissue-specific studies may require adjusted dosing strategies.

Recommended Approach

Given this variability, researchers should determine optimal dosing empirically for their specific mouse model, considering factors such as the target tissue, desired level of depletion, and experimental timeline.