Anti-Mouse Vγ2 TCR Purified In Vivo PLATINUM™

Anti-Mouse Vγ2 TCR – Purified in vivo PLATINUM™ Functional Grade

Product No.: T704


Clone UC3-10A6 Target Vγ2 TCR Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Vγ2 T-cell receptor, T cell receptor gamma 2 Isotype IgG Applications Depletion , FC , in vivo , IP


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Antibody Details Product Details Reactive Species Mouse Host Species Armenian Hamster Recommended Dilution Buffer In vivo Antibody Diluent pH 7.2 Immunogen G8 mouse T cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2832120 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry:It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 µg per 1.0 X 10⁶ cells in a 100 µl total staining volume, followed by Goat PN:A237. Additional Applications Reported In Literature ? Depletion: The UC3-10A6 antibody has been shown to deplete γδ T cells when administered in vivo. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Armenian Hamster Anti-Mouse Vγ2 TCR recognizes an epitope on Mouse Vγ2 TCR. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern. Background The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules. Clone UC3-10A6 antibody has been shown to deplete γδ T cells when administered in vivo. Antigen Distribution Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of γδ TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung. Ligand/Receptor Antigens PubMed Vγ2 TCR NCBI Gene Bank ID 110067 UniProt.org Information on Uniprot.org Research Area Adaptive Immunity . Immunology References & Citations 1. Hedrick, SM. et al. (1990) Nature. 343:714 View product citations for antibody T704 on CiteAb Technical Protocols Depletion Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Mouse

Host Species

Armenian Hamster

Recommended Dilution Buffer

In vivo Antibody Diluent pH 7.2

Immunogen

G8 mouse T cells

Product Concentration

≥ 5.0 mg/ml

Endotoxin Level

<0.5 EU/mg as determined by the LAL method

Purity

≥98% monomer by analytical SEC

⋅

>95% by SDS Page

Formulation

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Product Preparation

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Pathogen Testing

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Storage and Handling

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Country of Origin

USA

Shipping

Next Day 2-8°C

RRIDAB_2832120

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry:It is recommended to use the indirect method for signal enhancement when enumerating cells expressing TCR. A suggested method would be to stain cells expressing TCR with Anti-Mouse TCR at ≤1.0 µg per 1.0 X 10⁶ cells in a 100 µl total staining volume, followed by Goat PN:A237.

Additional Applications Reported In Literature ?

Depletion: The UC3-10A6 antibody has been shown to deplete γδ T cells when administered in vivo.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Armenian Hamster Anti-Mouse Vγ2 TCR recognizes an epitope on Mouse Vγ2 TCR. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern.

Background

The T cell receptor or TCR is a molecule found on the surface of T lymphocytes that is responsible for recognizing antigens bound to major histocompatibility complex (MHC) molecules. It is a heterodimer consisting of an α and β chain in 95% of T cells, while 5% of T cells have TCRs consisting of γ and δ chains. Engagement of the TCR with antigen and MHC results in activation of its T lymphocyte through a series of biochemical events mediated by associated enzymes, co-receptors and specialized accessory molecules. Clone UC3-10A6 antibody has been shown to deplete γδ T cells when administered in vivo.

Antigen Distribution

Vγ 2 T-Cell Receptor (TCR)-bearing T lymphocytes make up a significant proportion of γδ TCR-bearing cells in the late fetal and adult thymus and adult peripheral lymphoid tissues and lung.

Ligand/Receptor

Antigens

PubMed

Vγ2 TCR

NCBI Gene Bank ID

110067

UniProt.org

Information on Uniprot.org

Research Area

Adaptive Immunity

Immunology

References & Citations

1. Hedrick, SM. et al. (1990) Nature. 343:714

View product citations for antibody T704 on CiteAb

Depletion

Certificate of Analysis

Request COA

Formats Available

Prod No.	Description
T554	Anti-Mouse Vγ2 TCR – DyLight^® 488
T212	Anti-Mouse Vγ2 TCR – FITC
T219	Anti-Mouse Vγ2 TCR – APC
T353	Anti-Mouse Vγ2 TCR – Purified in vivo GOLD™ Functional Grade
T211	Anti-Mouse Vγ2 TCR – Biotin
T213	Anti-Mouse Vγ2 TCR – PE
T594	Anti-Mouse Vγ2 TCR – DyLight^® 650
T704	Anti-Mouse Vγ2 TCR – Purified in vivo PLATINUM™ Functional Grade
T210	Anti-Mouse Vγ2 TCR – Purified

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Anti-Mouse Vγ2 TCR – Purified in vivo PLATINUM™ Functional Grade