Anti-Mouse Vγ2 TCR – Purified in vivo PLATINUM™ Functional Grade

The UC3-10A6 clone is primarily used in in vivo mouse studies to selectively deplete ?? T cells expressing the V?2 T cell receptor. This depletion enables researchers to study the biological functions and immunological roles of these specific T cell populations in various contexts, such as infection, cancer, or tissue homeostasis.

Key supporting details:

• UC3-10A6 monoclonal antibody binds specifically to the V?2 (also called V?4) segment of the mouse TCR gamma chain, which is expressed on a major subset of ?? T cells in tissues including thymus, lymphoid organs, lung, intestinal epithelium, and epidermis.
• When administered to mice, UC3-10A6 is reported to cause in vivo depletion of V?2+ ?? T cells, mainly by mechanisms such as antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and antibody-dependent cellular phagocytosis (ADCP). This makes it a preferred tool for functional cell depletion studies in immunology.
• Typical in vivo applications include:
  • Assessing the contribution of ?? T cells to immune responses (e.g., infection or wound healing models).
  • Comparing the effects of antibody-mediated cell depletion to genetic knockout models.
  • Flow cytometric detection and sorting of V?2+ ?? T cells.
• Published research (such as in reference ) demonstrates the use of UC3-10A6 in experiments involving live mouse models, for both depletion and detection of V?2+ T cells.
• Protocols may vary, but the antibody is supplied as a high-purity, unconjugated Armenian hamster IgG and is administered via injection, with doses optimized depending on mouse strain, age, and experimental aim.

In summary, UC3-10A6 is used to selectively eliminate V?2+ ?? T cells in live mice, allowing researchers to dissect the immunological roles of these cells in vivo.

The correct storage temperature for sterile packaged clone UC3-10A6 depends on the intended storage duration.

• For short-term storage (up to 1 month): Store undiluted at 2–8°C (typically 4°C). Do not freeze.
• For long-term storage (months to a year): Aliquot and store at ?–20°C to –80°C, avoiding repeated freeze-thaw cycles.

Essential context and details:

• When stored at 4°C, the antibody remains stable for up to one month, or sometimes only 1–2 weeks as listed by some suppliers.
• For extended stability (up to one year), freezing at –20°C is advised; for even longer periods, store at –80°C.
• Always keep the solution undiluted and at the stock concentration in appropriate buffer.
• Avoid repeated freeze-thaw cycles as this can compromise antibody integrity.
• For functional-grade antibodies, aseptically aliquot into working volumes for freezing and do not dilute before freezing.

Summary of recommended storage conditions:

These guidelines align with those recommended by major suppliers for monoclonal antibody clone UC3-10A6.

Commonly used antibodies and proteins in conjunction with UC3-10A6 (anti-mouse V?2 TCR) in the literature typically include markers for T cell subsets, lineage markers, cytokines, and isotype controls for multi-parametric flow cytometry and immunophenotyping.

Key frequently co-used antibodies and proteins:

• Anti-MHC class II (M5/114.15.2)
• Anti-CD3? (145-2C11, 17A2) (total T cell marker)
• Anti-CD45.2 (104), Anti-CD45.1 (A20) (leukocyte markers and congenic strain discrimination)
• Anti-V?1 (2.11), Anti-V?4 (UC3-10A6), Anti-V?5 (536), Anti-V?6, Anti-V?7 (F2.67, 17D1) (other ?? T cell receptor chains for subset analysis)
• Anti-TCR? (GL3) (pan ? chain marker for ?? T cells)
• Anti-IL-17A (ebio17B7) (cytokine detection in T cells)
• Isotype controls (e.g., Hamster IgG HTK888, Mouse IgG1 MOPC-21, Mouse IgG2b eBMG2b) for validating specificity.

These antibodies are often used:

• In multiparametric flow cytometry panels to distinguish ?? T cell subsets and determine their phenotype.
• For in vivo depletion or functional studies, with controls like purified polyclonal Armenian hamster IgG.

Other commonly used proteins:

• Streptavidin (for biotin-labeled antibody detection).
• Anti-mouse IgG (Poly4053) (secondary detection or blocking).

The specific mix of antibodies and proteins chosen depends on the experimental objective, such as profiling immune populations, depleting cells, or analyzing cytokine expression, with different fluorochrome conjugates matched for compatibility in multicolor assays.

These combinatorial panels allow for precise analysis of the ?? T cell compartment, often comparing V?2-expressing cells with other TCR?? subsets and lineage or activation markers.

Clone UC3-10A6 is a monoclonal antibody that recognizes the mouse TCR V?2 segment and has been utilized in multiple research studies to investigate ?? T cell biology. Based on the available scientific literature, several key findings have emerged from studies using this clone.

?? T Cell Distribution and Function

The UC3-10A6 antibody has revealed that V?2 TCR-expressing T lymphocytes constitute a significant proportion of ?? T cells in various tissues. These cells make up a large portion of ?? T cells in late fetal and adult thymus, peripheral lymphoid tissues, lung, intestinal epithelium, and epidermis. The frequency of V?2 expression varies considerably between different mouse strains, ranging from 12% to 54% in the TCR ?? repertoire, with these variations not being associated with MHC haplotype.

Role in Malaria Infection

One of the most significant discoveries using UC3-10A6 involved identifying a specialized ?? T cell subset that produces macrophage colony-stimulating factor (M-CSF) during malaria infection. Research demonstrated that these M-CSF-producing ?? T cells fulfill a protective role specifically in the later stages of malaria infection when ?? T cells are involved.

During murine malaria studies, researchers discovered extensive clonal expansion of ?? T cells. At 21 days post-infection, infected animals showed 37 to 48 expanded clones among 193-270 successfully sequenced ?? T cells, compared to only one or two expanded clones in uninfected controls. Remarkably, approximately 75% of cells with identified TCR chains belonged to expanded clones in infected mice, versus only 2% in uninfected animals.

TCR Usage Patterns and Specificity

The studies revealed distinct patterns in TCR segment usage during infection. The ? chains of ?? T cells from infected animals predominantly used the TRAV15N-1 V-segment (also known as V?7 or V?6.3 in earlier nomenclatures), with an average of 74% of ?? T cell clones expressing this segment compared to only 15% in uninfected controls. Additionally, there was significant increase in TRGV1 gene segment usage and a decrease in TRGV4 usage in infected animals.

Tumor Biology Applications

Research has utilized UC3-10A6 to distinguish between different ?? T cell populations in cancer contexts. Studies found that most ?? T cells in premalignant or non-tumor colons exhibit cytotoxic markers, whereas tumor-infiltrating ?? T cells express protumorigenic characteristics, highlighting the dual role of these cells in cancer biology.

Immunological Surveillance Function

The antibody has contributed to understanding that TCR ?? T cells may play a crucial role in immunological surveillance for stress-induced self-antigens. These findings suggest that ?? T cells serve as sentinels that can detect cellular stress signals and respond accordingly.

Experimental Applications

UC3-10A6 has proven valuable as a research tool for in vivo depletion studies, as the antibody has been shown to effectively deplete ?? T cells when administered to experimental animals. This capability has made it an important reagent for functional studies investigating the role of ?? T cells in various disease models.

These findings collectively demonstrate that clone UC3-10A6 has been instrumental in advancing our understanding of ?? T cell biology, particularly regarding their roles in infection, cancer, and immune surveillance mechanisms.