Donkey F(ab')2 Anti-Rat IgG (H&L) (Adsorbed Against: Ms., Hu., Rb., Bv., Shp., Hs., GP., CK., Sy. Hms.) – DyLight® 650

Pricing & Details

  
Product Type
Secondary Antibody
Prod No.
Size
Price
Avail.
Qty
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R1306-0.5 mg
0.5 mg
$399.00
In stock
  

Antibody Details

Reactivity Species
Rat
Host Species
Donkey
Immunogen
Purified Rat IgG (H&L) (>98%)
Product Concentration
0.5 - 1.0 mg/ml
Formulation
This secondary antibody conjugate is formulated in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative.
Product Preparation
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Storage and Handling
This secondary antibody conjugate should be stored desiccated at 2-8°C.
Expiration Date
One year from date of receipt
Working Concentration
It is recommended that working dilutions be prepared fresh daily.

FC: 1:100 - 1:800
IF: 1:100 - 1:800

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5 - 1.0 µg/ml with a suitable second step reagent such as PN:G505. The detection sensitivity of this indirect ELISA for Mouse RANTES is approximately 0.5 ng/well.

Neutralization: The peptide may be used to neutralize the binding activity of PN:Z100 by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.

Western Blotting: To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:M114 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.
Specificity
Donkey F(ab')2 Anti-Rat IgG (H&L) recognizes Rat IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein Family
IgG (H&L)
  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.