NIH/3T3 Whole Cell Lysate

NIH/3T3 Whole Cell Lysate

Product No.: A195

[product_table name="All Top" skus="A195"]

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Product Type
Cell Line Lysate

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Details

Cell Line
NIH/3T3 (Embryo)
Tissue Type
Mouse Embryo
Growth Media
DMEM & 10% FBS (Fetal Bovine Serum)
Preparation
3T3 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Host
Mouse
Concentration
2.0 mg/ml
Formulation
The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.
Expiration Date
One year from date of receipt
Storage
This whole cell lysate is stable for three months at -20°C
Applications and Suggested Working Dilutions
This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.
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Prod No.
Description
M1036
M1049
R344
H568
M356
H665
H617
M389
R433
R389
M1356
M1358
M1359
M310
M1084
M287
R477
M300
R411
H744
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.