Recombinant Human MIP-1α

Home/Recombinant Proteins/Recombinant Human MIP-1α

Pricing & Details

Prod. No.M161
Expression Host
E. coli
Add to cart
10 µg
Min: 1
Step: 1
50 µg
Min: 1
Step: 1



Macrophage inflammatory protein 1 alpha (MIP-1α), also known as CCL3, is a member of the chemokine family (1). Its primary function is as a monocyte chemoattractant, but reports have also demonstrated chemoattractant and adhesive effects by MIP-1α on CD8 T cells, B cells and eosinophils. MIP-1α has been identified as a stem cell inhibitory factor that can block the proliferation of hematopoietic stem cells (2). The functional receptor for MIP-1α is CCR1 and CCR5 and it is has been shown to interact with CCL4 (3). Following acute lung injury in rats, MIP-1α stimulates production of TNF-alpha, which in turn up-regulates vascular adhesion molecules required for neutrophil influx (4). MIP-1α may have a potential role in host defense as a direct antiviral agent. It has been reported to show a significant direct antiviral activity against HSV-1(5).

Protein Details

>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human MIP-1α was determined by its ability to chemoattract cultured human monocytes or mouse BaF3 cells transfected with human CCR5. The expected ED50 for these effects are typically 2 - 10 ng/ml and 3 - 10 ng/ml, respectively.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
adtp taccfsytsr qipqnfiady fetssqcskp gvifltkrsr qvcadpseew vqkyvsdlel sa
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human MIP-1α is Mr 7.5 kDa.
This recombinant protein solutiion is supplied as a 0.2 μm filtered solution in 30% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.
Country of Origin
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Menten, P. et al. (2003) Cytokine Growth Factor Rev. 13:455
2. Cook, DN. et al. (1996) J. Leuko. Biol. 59:61
3. Kaufmann, A. et al. (2001) J. Leuko. Biol. 69:248
4. Ward, PA. et al. (1995) J. Immunol. 154:4793
5. Nakayama, T. et al. (2006) Virology 350:484
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Leinco Technologies uses cookies to improve your experience and our website service. By continuing to browse our website, you accept our cookie policy. Ok