Anti-Mouse MIP-1α – Biotin

Pricing & Details

  
Product Type
Polyclonal Antibody
Alternate Names
G0S19-1, LD78Alpha, MIP1A, SCYA3, CCL3
Applications
ELISA Det
,
WB
Prod No.
Size
Price
Avail.
Qty
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M1308-50 µg
50 µg
$425.00
In stock
  

Antibody Details

Reactivity Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse MIP-1α (Accession # Q5QNW0)
Formulation
This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present.
Storage and Handling
The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
Western Blotting: To detect Mouse MIP-1α this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Mouse MIP-1α is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M136.
ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/ml when used in conjunction with PN:M1233 as the capture antibody at 2-8 µg/ml and an optimal second step reagent such as PN:A106 for the detection of Mouse MIP-1α.

Description

Specificity
Goat Anti-Mouse Macrophage Inflammatory Protein 1 Alpha (MIP-1α) recognizes Mouse MIP-1α. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
Macrophage inflammatory protein 1 alpha (MIP-1 alpha) is a potent inhibitor of hemopoietic stem cell proliferation and is a member of a family of pro- inflammatory mediators, the chemokine family.1 MIP-1 alpha is a particularly interesting chemokine, because in addition to its proinflammatory activities, it inhibits the proliferation of hematopoietic stem cells.2 The role of MIP-1 alpha seems to be related to production of TNF-alpha, which in turn up-regulates vascular adhesion molecules required for neutrophil influx.3

Antigen Details

Protein
MIP-1 alpha
PubMed
MIP-1α+AND+Mouse
NCBI Gene Bank ID

References & Citations

1. Pragnell, IB. et al. (1994) J. Biol. Chem. 269: 4974
2. Cook, DN. et al. (1996) J. of Leukocyte Biol. 59: 61
3. Ward, PA. et al. (1995) J of Immunol. 154: 4793
Indirect Elisa Protocol
General Western Blot Protocol
  
Products are for research use only. Not for use in diagnostic or therapeutic procedures.