Anti-Human MIP-1α - Clone 93342 - Leinco Technologies

Pricing & Details


Product No.M154 Clone 93342 Protein MIP-1 alpha Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CCL3, LD78Alpha, MIP1A, SCYA3 Isotype Mouse IgG2b Applications CyTOF® , FC , WB Submit A Review	Printable Datasheet Printable SDS

Prod No.	Size	Price	Avail.	Qty	Add to cart
M154-25 µg	25 µg	$170.00	In stock	Max: Min: 1 Step: 1	Add to Cart
M154-100 µg	100 µg	$445.00	In stock	Max: Min: 1 Step: 1	Add to Cart
M154-500 µg	500 µg	$1,025.00	In stock	Max: Min: 1 Step: 1	Add to Cart


Antibody Details Product Details Reactivity Species Human Host Species Mouse Immunogen Purified Recombinant Human MIP-1α (Accession # P10147) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831358 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MIP-1α. A suggested method would be to stain cells expressing MIP-1α with 2.5 µg/10⁶ cells in a total staining volume of ≤200 µl followed by PN:M1189. Western Blotting: To detect Human MIP-1α this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MIP-1α is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:L145. Other Applications Reported In Literature ? CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Specificity Mouse Anti-Human Macrophage Inflammatory Protein 1 Alpha (MIP-1α) (Clone 93342) recognizes an epitope on Human MIP-1α. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background MIP-1α is a potent inhibitor of hemopoietic stem cell proliferation and is a member of a family of pro- inflammatory mediators, the chemokine family.¹ MIP-1α is a particularly interesting chemokine, because in addition to its proinflammatory activities, it inhibits the proliferation of hematopoietic stem cells.² The role of MIP-1α seems to be related to production of TNF-α, which in turn up-regulates vascular adhesion molecules required for neutrophil influx.³ Antigen Details Protein MIP-1 alpha PubMed MIP-1 alpha References & Citations 1. Pragnell, IB. et al. (1994) J. Biol. Chem. 269: 4974 2. Cook, DN. et al. (1996) J. of Leukocyte Biol. 59: 61 3. Ward, PA. et al. (1995) J of Immunol. 154: 4793 Technical Protocols

Antibody Details

Product Details

Reactivity Species

Human

Host Species

Mouse

Immunogen

Purified Recombinant Human MIP-1α (Accession # P10147)

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.

Storage and Handling

The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2831358

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing MIP-1α. A suggested method would be to stain cells expressing MIP-1α with 2.5 µg/10⁶ cells in a total staining volume of ≤200 µl followed by PN:M1189.
Western Blotting: To detect Human MIP-1α this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MIP-1α is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:L145.

Other Applications Reported In Literature ?

CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Mouse Anti-Human Macrophage Inflammatory Protein 1 Alpha (MIP-1α) (Clone 93342) recognizes an epitope on Human MIP-1α. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.

Background

MIP-1α is a potent inhibitor of hemopoietic stem cell proliferation and is a member of a family of pro- inflammatory mediators, the chemokine family.¹ MIP-1α is a particularly interesting chemokine, because in addition to its proinflammatory activities, it inhibits the proliferation of hematopoietic stem cells.² The role of MIP-1α seems to be related to production of TNF-α, which in turn up-regulates vascular adhesion molecules required for neutrophil influx.³

Antigen Details

Protein

MIP-1 alpha

PubMed

MIP-1 alpha

References & Citations

1. Pragnell, IB. et al. (1994) J. Biol. Chem. 269: 4974
2. Cook, DN. et al. (1996) J. of Leukocyte Biol. 59: 61
3. Ward, PA. et al. (1995) J of Immunol. 154: 4793

Prod No.	Description
M154	Anti-Human MIP-1α – Clone 93342
M151	Anti-Rat MIP-1α
C1418	Anti-Mouse MIP-1α – Clone 39624
C1447	Anti-Human MIP-1α – Clone 14215
C1390	Anti-Human MIP-1α – Clone 93321
M147	Anti-Human MIP-1α
M177	Anti-Mouse MIP-1α
L145	Recombinant Human MIP-1α (CCL3L1)
M161	Recombinant Human MIP-1α
M136	Recombinant Mouse MIP-1α
M1308	Anti-Mouse MIP-1α – Biotin

Formats Available

Prod No.	Description
M154	Anti-Human MIP-1α – Clone 93342

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

Anti-Human MIP-1α – Clone 93342