Anti-Human MIP-1α – Clone 93321

Pricing & Details

  
Clone
93321
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CCL3, G0S19-1, LD78Alpha, MIP1A, SCYA3,
Isotype
Mouse
IgG1
Applications
ELISA Indirect
,
IHC FFPE
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1390-25 µg
25 µg
$175.00
In stock
C1390-100 ug
100 ug
$339.00
In stock
C1390-500 µg
500 µg
$750.00
In stock
  

Antibody Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Purified Recombinant Human MIP-1α (Accession # P10147)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
See directions on lot specific datasheets, as information may periodically change.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
Indirect ELISA: This antibody can be used in an indirect detection ELISA at 0.5-1.0 µg/ml with a suitable second step reagent such as PN:M114. The detection sensitivity of this indirect ELISA for Human CCL3 is approximately 3.0 ng/well.
IHC (NBF/Par.): This antibody should give satisfactory staining results when used at a concentration of 25 µg/ml. The recommended secondary antibody for IHC is PN:M114. For chromogenic detection with high signal and low background use PN:D100 or PN:K107.
Neutralization: This antibody is useful for neutralization of Human CCL3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Human CCL3 (at 100 ng/ml) is 0.5-1.5 µg/ml.
Western Blotting: To detect Human MIP-1α this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human MIP-1α is 20 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:L145.
Each investigator should determine their own optimal working dilution for specific applications.

Description

Specificity
Mouse Anti-Human Macrophage Inflammatory Protein 1 Alpha (MIP-1α) (Clone 93321) recognizes an epitope on Human MIP-1α. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Macrophage inflammatory protein 1 alpha (MIP-1 alpha) is a potent inhibitor of hemopoietic stem cell proliferation and is a member of a family of pro- inflammatory mediators, the chemokine family.1 MIP-1 alpha is a particularly interesting chemokine, because in addition to its proinflammatory activities, it inhibits the proliferation of hematopoietic stem cells.2 The role of MIP-1 alpha seems to be related to production of TNF-alpha, which in turn up-regulates vascular adhesion molecules required for neutrophil influx.3

Antigen Details

Protein
MIP-1 alpha

References & Citations

1. Pragnell, IB. et al. (1994) J. Biol. Chem. 269: 4974
2. Cook, DN. et al. (1996) J. of Leukocyte Biol. 59: 61
3. Ward, PA. et al. (1995) J of Immunol. 154: 4793
Indirect Elisa Protocol
IHC
General Western Blot Protocol
  

Formats Available

  
Prod No.
Description
C1390
Products are for research use only. Not for use in diagnostic or therapeutic procedures.