Anti-Human MIP-1α – Clone 14215

Anti-Human MIP-1α – Clone 14215

Product No.: C1447

[product_table name="All Top" skus="C1447"]

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Clone
14215
Target
MIP-1 alpha
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CCL3, G0S19-1, LD78Alpha, MIP1A, SCYA3
Isotype
IgG2a
Applications
ELISA
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N

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
E. coli-Derived Recombinant Human CCL3
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
Additional Applications Reported In Literature ?
Neutralization: The peptide may be used to neutralize the binding activity of PN:T361 by incubation of an equal volume of the peptide and antibody for 30 minutes at 37°C for Western blotting experiments.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Macrophage Inflammatory Protein-1α (MIP-1α) (Clone 14215) recognizes an epitope on Human MIP-1α. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Macrophage inflammatory protein 1 alpha (MIP-1 alpha) is a potent inhibitor of hemopoietic stem cell proliferation and is a member of a family of pro- inflammatory mediators, the chemokine family.1 MIP-1 alpha is a particularly interesting chemokine, because in addition to its proinflammatory activities, it inhibits the proliferation of hematopoietic stem cells.2 The role of MIP-1 alpha seems to be related to production of TNF-alpha, which in turn up-regulates vascular adhesion molecules required for neutrophil influx.3

Antigen Details

NCBI Gene Bank ID
Research Area
Other Molecules

References & Citations

1. Pragnell, IB. et al. (1994) J. Biol. Chem. 269: 4974
2. Cook, DN. et al. (1996) J. of Leukocyte Biol. 59: 61
3. Ward, PA. et al. (1995) J of Immunol. 154: 4793
Indirect Elisa Protocol
N

Certificate of Analysis

Formats Available

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Prod No.
Description
C1447
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.