Anti-Mouse MIP-1α – Clone 39624

Pricing & Details

  
Clone
39624
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
G0S19-1, LD78Alpha, MIP1A, SCYA3, CCL3
Isotype
Rat
IgG2a
Applications
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1418-25 µg
25 µg
$155.00
In stock
C1418-100 µg
100 µg
$339.00
In stock
C1418-500 µg
500 µg
$785.00
In stock
  

Antibody Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse MIP-1α (>98%)
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Formulation
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
See directions on lot specific datasheets, as information may periodically change.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
Neutralization: This antibody is useful for neutralization of Mouse CCL3 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse CCL3 (at 100 ng/ml) is 5 - 25 µg/ml.
Western Blotting: To detect Mouse MIP-1α this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse MIP-1α is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M136.
Each investigator should determine their own optimal working dilution for specific applications.

Description

Specificity
Rat Anti-Mouse Macrophage Inflammatory Protein 1 Alpha (MIP-1α) (Clone 39624) recognizes an epitope on Mouse MIP-1α. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Macrophage inflammatory protein 1 alpha (MIP-1 alpha) is a potent inhibitor of hemopoietic stem cell proliferation and is a member of a family of pro- inflammatory mediators, the chemokine family.1 MIP-1 alpha is a particularly interesting chemokine, because in addition to its proinflammatory activities, it inhibits the proliferation of hematopoietic stem cells.2 The role of MIP-1 alpha seems to be related to production of TNF-alpha, which in turn up-regulates vascular adhesion molecules required for neutrophil influx.3

Antigen Details

Protein
MIP-1 alpha

References & Citations

1. Pragnell, IB. et al. (1994) J. Biol. Chem. 269: 4974
2. Cook, DN. et al. (1996) J. of Leukocyte Biol. 59: 61
3. Ward, PA. et al. (1995) J of Immunol. 154: 4793
General Western Blot Protocol
  

Formats Available

  
Prod No.
Description
C1418
Products are for research use only. Not for use in diagnostic or therapeutic procedures.