Recombinant Mouse MIP-1α

Pricing & Details

Product. No.M136
Expression Host
E. coli
Reactivity Species
Mouse
Prod No.
Size
Price
Avail.
Qty
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M136-10 µg
10 µg
$279.00
In stock
Max:
Min: 1
Step: 1
M136-50 µg
50 µg
$429.00
In stock
Max:
Min: 1
Step: 1

Background

Macrophage inflammatory protein 1 alpha (MIP-1 alpha) is a potent inhibitor of hemopoietic stem cell proliferation and is a member of a family of pro- inflammatory mediators, the chemokine family.1 MIP-1 alpha is a particularly interesting chemokine, because in addition to its proinflammatory activities, it inhibits the proliferation of hematopoietic stem cells.2 The role of MIP-1 alpha seems to be related to production of TNF-alpha, which in turn up-regulates vascular adhesion molecules required for neutrophil influx.3

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Mouse MIP-1α was determined by its ability to chemoattract cultured human monocytes or mouse BaF3 cells transfected with human CCR5. The expected ED50 for these effects are typically 5 - 25 ng/ml and 0.4 - 2.0 ng/ml, respectively.
Expression Host
E. coli
Protein Accession No.
Q5QNW0
Amino Acid Sequence
apygadtpta ccfsysrkip rqfivdyfet sslcsqpgvi fltkrnrqic adsketwvqe yitdlelna
N-terminal Seqence Analysis
Ala24
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Mouse MIP-1α is Mr 7.8 kDa.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
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References & Citations

1. Pragnell, IB. et al. (1994) J. Biol. Chem. 269: 4974
2. Cook, DN. et al. (1996) J. of Leukocyte Biol. 59: 61
3. Ward, PA. et al. (1995) J of Immunol. 154: 4793
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.