Syrian Hamster IgG Isotype Control — Purified in vivo GOLD™ Functional Grade

Syrian Hamster IgG Isotype Control — Purified in vivo GOLD™ Functional Grade

Product No.: I-444

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Clone
Polyclonal
Formats AvailableView All
Product Type
Polyclonal Isotype Control
Isotype
IgG
Applications
FA
,
FC
,
in vivo

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Select Product Size
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Antibody Details

Product Details

Host Species
Syrian Hamster
Recommended Dilution Buffer
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC This isotype control antibody should be used at the same concentration as the primary antibody.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This isotype control antibody is for use as a negative control in the measurement of the non-specific binding of Syrian hamster monoclonal antibodies of isotype IgG to human, mouse or rat tissues.

Leinco Antibody Advisor

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Polyclonal antibodies are commonly used in in vivo mouse studies to target and inhibit specific tumor growths, study immune responses, or evaluate therapeutic interventions. In these studies, polyclonal antibodies, which are a mixture of immunoglobulins recognizing multiple epitopes on a target antigen, can be generated against various murine or human tumor antigens, pathogens, or cell populations.

Key uses and methodologies include:

  • Tumor inhibition and therapeutic assessment:
    Polyclonal antibodies generated against specific murine tumor antigens (e.g., melanoma, hepatocellular carcinoma, colorectal cancer) are injected into mice—either alone or co-administered with immune checkpoint inhibitors—to test their ability to inhibit tumor growth in syngeneic models. For example, rabbit polyclonal antibodies were used in orthotopic and heterotopic tumor models in C57BL/6 and Balb/c mice, demonstrating selective inhibition of tumor progression when administered intraperitoneally on a biweekly schedule.

  • Humanized mouse models for polyclonal repopulation studies:
    In studies of hematopoietic stem cell transplantation in humanized BLT (bone marrow/liver/thymus) mice, polyclonal repopulation patterns are tracked to understand clonal expansion and stem cell therapy outcomes. Techniques like low-volume vector integration site sequencing (LoVIS-Seq) enable longitudinal tracking of the fate and diversity of polyclonal human hematopoietic clones in vivo.

  • Production and validation of human polyclonal antibodies:
    Transgenic approaches (e.g., TransChromo technology) introduce large segments of human immunoglobulin loci into mice to produce fully human polyclonal antibodies upon immunization with specific antigens. These antibodies can be tested for efficacy against pathogens or as therapeutic agents in mouse challenge models, providing a platform to assess their potential for clinical translation.

  • Treatment protocols in tumor models:
    In cancer studies, treatment with polyclonal antibodies typically starts several days after tumor cell injection and is carried out for periods (e.g., 28 days), with dosage and regimen tailored for efficacy and comparison against controls. Tumor growth and survival are monitored, alongside clinical scores to assess general health.

In summary, polyclonal antibodies in in vivo mouse studies are pivotal for evaluating therapeutic potential in cancer and infectious disease models, as well as understanding clonal dynamics in humanized systems. Their broad antigen recognition profile makes them valuable for targeting heterogeneous disease epitopes and for preclinical testing of antibody therapies.

For the correct storage temperature of a sterile packaged polyclonal antibody, it is generally recommended to store it at -20°C. This temperature helps maintain the stability and activity of the antibody over long periods, although some sources suggest that storing at this temperature is not necessary if the antibodies are not subject to repeated freeze-thaw cycles and are stored in high concentrations.

For short-term storage, up to a few weeks, 4°C is acceptable, but the use of preservatives like sodium azide is recommended to prevent microbial growth. However, it is always best to consult the specific instructions provided by the manufacturer for optimal storage conditions.

Here is a summary:

  • Long-term storage: -20°C
  • Short-term storage: 4°C (with preservatives like sodium azide)

Key Considerations

  • Avoid Freeze-Thaw Cycles: These can damage antibodies.
  • Preservatives: Use sodium azide if storing at 4°C.
  • Check Manufacturer’s Instructions: Always refer to the datasheet for specific recommendations.

Based on the literature, polyclonal antibodies are commonly used alongside or compared with several other types of antibodies and proteins in research applications.

Monoclonal Antibodies

Monoclonal antibodies represent the most frequently referenced comparison to polyclonal antibodies in scientific literature. While polyclonal antibodies recognize multiple epitopes on the same target protein, monoclonal antibodies are highly specific and detect only one epitope on the antigen. This fundamental difference makes them complementary tools in research, with studies often comparing their effectiveness for specific applications like Western blotting, ELISA, immunohistochemistry, and immunofluorescence assays.

Recombinant Antibodies

Recombinant antibodies are another class frequently discussed alongside polyclonal antibodies. These engineered antibodies offer advantages in terms of reproducibility and scalability, addressing some of the batch-to-batch variability challenges inherent in traditional polyclonal antibody production.

Therapeutic Antibodies in Cancer Research

In oncology applications, polyclonal antibodies are studied in conjunction with specific therapeutic targets:

HER1/EGFR and HER2 antibodies - Research demonstrates that polyclonal antibodies targeting these receptors can surpass the effectiveness of registered monoclonal antibody combinations. Studies compare vaccination-induced polyclonal antibodies against established therapeutic monoclonal antibodies like cetuximab for cancer treatment.

Tyrosine-kinase inhibitors (TKIs) are also frequently mentioned alongside polyclonal antibodies in cancer research, as both represent different approaches to targeting the same cellular pathways.

Secondary Detection Antibodies

Polyclonal antibodies are commonly used with secondary antibodies in various immunoassays. These secondary antibodies can be generated in different species including rabbit, goat, sheep, donkey, and chicken, providing researchers with multiple options for experimental design.

Antibody Fragments

The literature also references various antibody fragments and engineered antibody constructs that can be derived from polyclonal sources through sequencing and cloning approaches. These include single-chain variable fragments (scFv) and other recombinant formats that maintain the binding characteristics of the original polyclonal mixture.

The integration of polyclonal antibodies with these other protein tools often depends on the specific research application, with each type offering distinct advantages for particular experimental conditions and research objectives.

Key findings from scientific literature on clone polyclonal citations primarily revolve around the distinct biological properties, research applications, and evolutionary significance of polyclonality versus monoclonality in both antibodies and tumorigenesis.

Major Findings:

  • Polyclonality in Antibody Research:

    • Polyclonal antibodies are produced from multiple B-cell clones, resulting in a mixture that recognizes multiple epitopes on an antigen.
    • Their diversity can enhance signal detection, making them valuable for immunoassays that require high sensitivity, such as Western blotting, ELISA, and immunoprecipitation.
    • Sequencing of polyclonal antibodies enables researchers to analyze clonal diversity, V(D)J usage, and somatic hypermutation patterns, providing insight into immune response dynamics and antibody population structure.
    • Recombinant polyclonal antibody technology allows for renewable, highly diverse polyclonal mixtures, overcoming limits of traditional animal-derived polyclonals and facilitating large-scale production.
    • Variations among batches of identical polyclonal antibodies may occur due to changes in the underlying clone populations, leading to differences in signal intensity and background levels; sequencing helps clarify and control these batch effects.
  • Polyclonality in Tumor Evolution:

    • Schematic lineage tracing and genomic studies have demonstrated that many tumors, particularly in early colorectal cancer, originate polyclonally from multiple clones, with smaller and less malignant lesions.
    • A key discovery is the polyclonal-to-monoclonal transition model, which posits that early, benign tumors are polyclonal in origin; as malignancy increases, a dominant clone emerges, resulting in a monoclonal tumor with higher genomic instability and aggressiveness.
    • Genomic studies on patient samples reveal that approximately 30% of colorectal polyps show polyclonal origins associated with lower mutational burdens and copy number variation, confirming preclinical findings.
    • These findings suggest that monitoring clonal dynamics and targeting cell–cell interactions may open new avenues for early cancer intervention.

Additional Relevant Points:

  • Technical Advances:

    • Integration of next-generation sequencing (NGS) with mass spectrometry (MS) has improved precision in antibody clone identification and quantification, enabling better intellectual property protection and functional characterization.
    • Clonal composition sequencing supports recombinant antibody engineering, structural modeling, and identification of immunodominant responses for vaccine or therapeutic development.
  • Comparative Summary:

Feature/Key IssuePolyclonal (Antibody/Tumor)Monoclonal (Antibody/Tumor)
SourceMultiple B-cell clones/multiple tumor cell lineagesSingle B-cell clone/single tumor cell lineage
Epitope coverageMultipleSingle
Signal strength/sensitivityHighLower (but specific)
SpecificityLower (risk of cross-reactivity)High
Production cost/timeLower, fasterHigher, slower
Malignancy (tumors)LowerHigher
Research applicationImmunoprecipitation, Western blot, ELISADiagnostics, therapeutics, precision research

Scientific literature thus highlights that clonal polyclonal analysis provides vital understanding for immunoassay development, cancer progression modeling, and advances in recombinant biotechnology, supporting both basic research and clinical innovation.

References & Citations

FA
Flow Cytometry
in vivo Protocol

Certificate of Analysis

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.