Anti-Human CD151 – Purified in vivo GOLD™ Functional Grade
Anti-Human CD151 – Purified in vivo GOLD™ Functional Grade
Product No.: C2039
Clone 50-6 Target CD151 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names PETA-3, Platelet-Endothelial tetra-span antigen, Tspan-24 Isotype Mouse IgG1 κ Applications B , CyTOF® , FC , ICC , in vivo , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Human epidermoid carcinoma cell line HEp-3 Product Concentration ≥ 2.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829117 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 50-6 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? B CyTOF® ICC WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 50-6 recognizes an epitope on human CD151. Background CD151 is a 29 kD cell surface glycoprotein that is a member of the transmembrane 4 superfamily (tetraspanin family), which is characterized by cell-surface proteins consisting of four hydrophobic domains that mediate signal transduction events involved in the regulation of cell development, activation, growth and motility. CD151 is known to complex with integrins and other transmembrane 4 superfamily proteins and is involved in cell adhesion, migration, and tumor cell metastasis. Antigen Distribution CD151 is found on immature hematopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells, vascular endothelium, and activated T-cells. Ligand/Receptor CD9, CD181, integrins α3β1, α5β1 and α6β4 Function Cell adhesion, migration NCBI Gene Bank ID UniProt.org Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone 50-6 is a monoclonal antibody that targets CD151, and in in vivo mouse studies, it is primarily used to inhibit metastasis of human tumor cells by binding to the CD151 antigen, which is associated with cell migration and metastasis. In these studies:
Summary of typical use in mouse studies:
This antibody is an example of an in vivo functional grade primary antibody validated for use in mouse models, specifically targeting CD151 to study its role in tumor progression and metastasis. Commonly used antibodies or proteins used with 50-6 (an anti-CD151 monoclonal antibody) in the literature typically depend on the context of the experiment, but there are several that are regularly paired in immunophenotyping or cancer/epithelial biology studies.
Flow cytometry and immunofluorescence panels that include 50-6 will almost always include multiple lineage or functional antibodies to allow multiparameter analysis, consistent with best practices in antibody-based research. If you are seeking specific combinations reported in primary literature, referencing articles or protocols where the 50-6 clone is cited often reveals that it appears together with markers relevant to the cell type or biological process of interestmost frequently integrins, other tetraspanins, and cell lineage markers. If you want a precise list from a particular context (e.g., a particular tissue or disease), more specific details can be provided. OverviewThe term "clone 50-6" is not directly referenced in the provided search results, and there is no scientific analysis or paper in the first several pages referencing a specific "clone 50-6" by that identifier. However, results regarding clone pSC101 are discussed in the context of seminal work in DNA cloningspecifically, as recounted by Stanley N. Cohen in his personal account. If your query refers to a specific "clone 50-6" from a particular organism, system, or technology (e.g., gene, cell line, or code clone), the provided search does not contain that information. Below, I summarize the most relevant scientific findings related to DNA cloning (Cohen, 1973), which many associate with key early clones like pSC101a foundational cloning vectorand explain the significance of those findings. Key Scientific Findings in Early DNA Cloning
Limitations of the SearchIf "clone 50-6" refers to a specific entity not related to the Cohen–Boyer DNA cloning experiments (e.g., a cell line, a particular clone in a laboratory organism such as yeast or zebrafish, or a code clone in computer science), the cited literature does not contain information about it. The only clone-related scientific findings described in the search are those related to pSC101 and the origins of DNA cloning technology. ConclusionIf "clone 50-6" is a unique identifier from your research context, you must specify the organism, system, or field (e.g., molecular biology, immunology, cell biology, or software engineering) for targeted information. If you are referring to the foundational DNA cloning work, the key findings are the establishment of pSC101 as a cloning vector, the demonstration of gene cloning using restriction enzymes, and the feasibility of propagating foreign DNA in bacterial cellsmilestones that revolutionized molecular biology. There is no published evidence in the provided results detailing the dosing regimens of clone 50-6 across mouse models. None of the cited literature or antibody guides specifically mention clone 50-6, its target, or its pharmacological context in vivo. Thus, an evidence-based answer about dose, frequency, or variation between models for clone 50-6 is not possible from the available search data. If clone 50-6 refers to a commonly used research antibody, its dosing regimen would typically be informed by:
For comparable monoclonal antibodies in mouse models:
Since no information about clone 50-6 was present in the search results, any recommendation beyond the above generalization would be speculative. For precise guidance, refer to the product datasheet from the antibody supplier or relevant peer-reviewed studies using clone 50-6. If you provide the target or context for clone 50-6 (such as antigen, company, or disease model), a more precise or inferential dosing recommendation may be possible. References & Citations1. Testa, et. al., Cancer Research. 59:3812-3820, 1999 Technical ProtocolsCertificate of Analysis |
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