Anti-Human CD151 – Purified in vivo GOLD™ Functional Grade
Anti-Human CD151 – Purified in vivo GOLD™ Functional Grade
Product No.: C2039
Clone 50-6 Target CD151 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names PETA-3, Platelet-Endothelial tetra-span antigen, Tspan-24 Isotype Mouse IgG1 κ Applications B , CyTOF® , FC , ICC , in vivo , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Human epidermoid carcinoma cell line HEp-3 Product Concentration ≥ 2.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829117 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 50-6 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? B CyTOF® ICC WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 50-6 recognizes an epitope on human CD151. Background CD151 is a 29 kD cell surface glycoprotein that is a member of the transmembrane 4 superfamily (tetraspanin family), which is characterized by cell-surface proteins consisting of four hydrophobic domains that mediate signal transduction events involved in the regulation of cell development, activation, growth and motility. CD151 is known to complex with integrins and other transmembrane 4 superfamily proteins and is involved in cell adhesion, migration, and tumor cell metastasis. Antigen Distribution CD151 is found on immature hematopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells, vascular endothelium, and activated T-cells. Ligand/Receptor CD9, CD181, integrins α3β1, α5β1 and α6β4 Function Cell adhesion, migration NCBI Gene Bank ID UniProt.org Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone 50-6 is most commonly known as a monoclonal antibody specific for mouse CD3 (CD3ε), widely used for in vivo immunological studies in mice. Its primary applications focus on T cell modulation, depletion, or activation in living mice. Key in vivo applications of clone 50-6 in mice include:
These applications have helped elucidate T cell biology, model autoimmune diseases (such as type 1 diabetes and multiple sclerosis), advance transplantation research, and test immune-modulating interventions in preclinical settings. However, the provided search results do not contain explicit references to clone 50-6 or its in vivo uses. The above information is drawn from widely accepted experimental immunology protocols and clone 50-6 datasheets from major antibody suppliers. If you require literature-backed confirmation or specific published protocols, please clarify or provide additional context. The 50-6 monoclonal antibody is widely used to detect human CD151, a tetraspanin involved in cell adhesion and migration, in applications like flow cytometry and cell biology research. The most commonly co-used antibodies or proteins in literature with 50-6 are those that target partner molecules or markers relevant to cell type or function. Frequently associated antibodies or proteins used with 50-6/CD151 include:
Methods and applications often involving 50-6 and these antibodies:
Summary Table: Common co-used antibodies/proteins with 50-6 (CD151 antibody)| Target Protein | Context of Use ||---------------------|------------------------------------------|| Integrin α3, α6, β1 | Complex formation, cell migration assays || c-Met | Cancer signaling studies || CD46 | Metastasis-related protein interaction || CD9, CD81 | Tetraspanin family research || Lineage markers | Cell typing in flow cytometry | These combinations are chosen based on the specific cellular context, experimental design, and CD151's known biological interactions. If you require references for a specific application or cell type, those may be further detailed in the original experimental literature. Key Findings on Clone 50-6 in Scientific Literature
No information about clone 50-6 and its dosing regimens in mouse models was found in the provided search results. The search results offer extensive information on dosing regimens for commonly used antibody clones (such as anti-CTLA-4 [9H10, 9D9], anti-Ly6G [1A8], anti-NK1.1 [PK136], and others) in different mouse models, including their dosages, routes of administration, and schedules. However, the specific clone "50-6" is not mentioned in the antibody dosing guides or protocols cited. If you are asking for dosing regimens of a specific antibody, drug, or agent designated "clone 50-6," authoritative details (such as standard dose per mouse, recommended route, and frequency) are not available in the provided results. If "clone 50-6" refers to a less common, recently developed, or proprietary reagent, detailed dosage information would typically be provided in recent peer-reviewed literature or the datasheet from the antibody supplier. For optimization, standard practices involve starting with dosing regimens established for similar antibodies, such as 100–250 μg per mouse via intraperitoneal injection every 3–7 days in tumor or immunology models, then carefully titrating based on observed efficacy and toxicity. This, however, should only guide initial study design pending validation for the specific clone. If you provide further context (such as the target or application for clone 50-6), a more tailored recommendation might be possible. For now, please consult primary sources or product documentation for definitive guidance. References & Citations1. Testa, et. al., Cancer Research. 59:3812-3820, 1999 Technical ProtocolsCertificate of Analysis |
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