Anti-Human CD151 – Purified In vivo GOLD™ Functional Grade

Pricing & Details

Product No.C2039
Clone
50-6
Protein
CD151
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
PETA-3, Platelet-Endothelial tetra-span antigen, Tspan-24
Isotype
Mouse
IgG1 κ
Applications
B
,
CyTOF®
,
FC
,
ICC
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2039-500ug
500 µg
$355.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Human epidermoid carcinoma cell line HEp-3
Product Concentration
≥ 2.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 50-6 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Other Applications Reported In Literature ?
B
CyTOF®
ICC
WB
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 50-6 recognizes an epitope on human CD151.
Antigen Distribution
CD151 is found on immature hematopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells, vascular endothelium, and activated T-cells.
Background
CD151 is a 29 kD cell surface glycoprotein that is a member of the transmembrane 4 superfamily (tetraspanin family), which is characterized by cell-surface proteins consisting of four hydrophobic domains that mediate signal transduction events involved in the regulation of cell development, activation, growth and motility. CD151 is known to complex with integrins and other transmembrane 4 superfamily proteins and is involved in cell adhesion, migration, and tumor cell metastasis.

Antigen Details

Protein
Ligand/Receptor
CD9, CD181, integrins α3β1, α5β1 and α6β4
Function
Cell adhesion, migration
PubMed
NCBI Gene Bank ID

References & Citations

1. Testa, et. al., Cancer Research. 59:3812-3820, 1999
Flow Cytometry
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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