Anti-Human CD151 – Purified in vivo GOLD™ Functional Grade
Pricing & Details
Antibody DetailsProduct DetailsReactivity Species Human Host Species Mouse Immunogen Human epidermoid carcinoma cell line HEp-3 Product Concentration ≥ 2.0 mg/ml Endotoxin Level ≤ 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829117 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 50-6 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. Other Applications Reported In Literature ? B CyTOF® ICC WB Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone 50-6 recognizes an epitope on human CD151. Antigen Distribution CD151 is found on immature hematopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells, vascular endothelium, and activated T-cells. Background CD151 is a 29 kD cell surface glycoprotein that is a member of the transmembrane 4 superfamily (tetraspanin family), which is characterized by cell-surface proteins consisting of four hydrophobic domains that mediate signal transduction events involved in the regulation of cell development, activation, growth and motility. CD151 is known to complex with integrins and other transmembrane 4 superfamily proteins and is involved in cell adhesion, migration, and tumor cell metastasis. Antigen DetailsProtein Ligand/Receptor CD9, CD181, integrins α3β1, α5β1 and α6β4 Function Cell adhesion, migration PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Testa, et. al., Cancer Research. 59:3812-3820, 1999 Technical Protocols |
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