Anti-Human CD151 – Purified in vivo GOLD™ Functional Grade

Anti-Human CD151 – Purified in vivo GOLD™ Functional Grade

Product No.: C2039

[product_table name="All Top" skus="C2039"]

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Clone
50-6
Target
CD151
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
PETA-3, Platelet-Endothelial tetra-span antigen, Tspan-24
Isotype
Mouse IgG1 κ
Applications
B
,
CyTOF®
,
FC
,
ICC
,
in vivo
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Recommended Dilution Buffer
Immunogen
Human epidermoid carcinoma cell line HEp-3
Product Concentration
≥ 2.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 50-6 antibody for staining cells in flow cytometry is ≤ 1 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
B
CyTOF®
ICC
WB
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone 50-6 recognizes an epitope on human CD151.
Background
CD151 is a 29 kD cell surface glycoprotein that is a member of the transmembrane 4 superfamily (tetraspanin family), which is characterized by cell-surface proteins consisting of four hydrophobic domains that mediate signal transduction events involved in the regulation of cell development, activation, growth and motility. CD151 is known to complex with integrins and other transmembrane 4 superfamily proteins and is involved in cell adhesion, migration, and tumor cell metastasis.
Antigen Distribution
CD151 is found on immature hematopoietic cells, megakaryocytes, platelets, keratinocytes, epithelial cells, muscle cells, Schwann cells, vascular endothelium, and activated T-cells.
Ligand/Receptor
CD9, CD181, integrins α3β1, α5β1 and α6β4
Function
Cell adhesion, migration
NCBI Gene Bank ID

References & Citations

1. Testa, et. al., Cancer Research. 59:3812-3820, 1999
B
CyTOF®
Flow Cytometry
ICC
in vivo Protocol
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.