Anti-Human CD18 (ITGB2) – APC
Anti-Human CD18 (ITGB2) – APC
Product No.: C932
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Clone IB4 Target CD18 Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names Integrin beta-2, integrin beta chain, beta 2, ITGB2 Isotype IgG2a κ Applications FA , FC , IF Microscopy |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Human peripheral blood lymphocytes Product Concentration 0.2 mg/ml Formulation This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. State of Matter Liquid Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling This Allophycocyanin (APC) conjugate is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only Country of Origin USA Shipping 2-8°C Wet Ice Excitation Laser Red Laser (650 nm) Applications and Recommended Usage? Quality Tested by Leinco FC, IHC, IP Additional Applications Reported In Literature ? IF Microscopy, FA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity IB4 activity is directed against human CD18 and can also stain rabbit bone marrow leukocytes. Background Integrins are a large family of α/β heterodimeric transmembrane molecules that mediate adhesion, migration, cell survival, and cell differentiation1. The leukocytic cell-adhesion molecule (Leu-CAM) family has three members, LFA-1 (CD18/CD11a), CR3 (CD18/CD11b), and p150,95 (CD18/CD11c), all of which share the β chain CD182.
Murine (m) 1B4 was humanized (h) by the CDR-grafting method to create an anti-CD18 antibody with similar avidity, specificity, and efficacy as the murine antibody3. In rhesus monkeys, h1B4 is less immunogenic than m1B4 and elicits only anti-idiotypic antibodies. Additionally, h1B4 and m1B4 exhibit equivalent staining specificity and intensity in rabbit bone marrow leukocytes. For humanization, human VH and VL FR were chosen based on high identity with their corresponding m1B4 FR3. Once grafted with the m1B4 CDR, the human VH and VL FR were expressed as fusion proteins with the IgG4 and Cκ human constant regions, respectively. The h1B4 monoclonal antibody has been used in multiple studies of adhesion in different model systems4, 5, 6 and to measure the expression of CD18 in B-ALL cell lines7. Antigen Distribution Depending on its heterodimeric configuration, CD18 is expressed in leukocytes, monocytes, macrophages, some lymphocytes, polymorphonuclear lymphocytes (PMNs), natural killer cells, neutrophils, granulocytes, certain lymphocytic tumor cell lines, macrophage foam cells, activated platelets, and splenic red pulp macrophages. Ligand/Receptor Receptor NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Immunology . Signal Transduction References & Citations1. Luo BH, Carman CV, Springer TA. Annu Rev Immunol. 25:619-647. 2007.
2. Sanchez-Madrid F, Nagy JA, Robbins E, et al. J Exp Med. 158(6):1785-1803. 1983. 3. Singer II, Kawka DW, DeMartino JA, et al. J Immunol. 150(7):2844-2857. 1993. 4. Hanlon WA, Stolk J, Davies P, et al. J Leukoc Biol. 50(1):43-48. 1991. 5. Seth R, Salcedo R, Patarroyo M, et al. FEBS Lett. 282(1):193-196. 1991. 6. McDonald RJ, St George JA, Pan LC, et al. Inflammation. 17(2):145-151. 1993. 7. Velázquez-Avila M, Balandrán JC, Ramírez-Ramírez D, et al. Leukemia. 33(6):1337-1348. 2019. Technical ProtocolsCertificate of Analysis |
Formats Available
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Prod No. | Description |
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C932 | |
C933 | |
C934 | |
C935 | |
C939 | |
C940 | |
C941 | |
C942 | |
C930 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.