Anti-Human CD62L – Purified in vivo PLATINUM™ Functional Grade
Anti-Human CD62L – Purified in vivo PLATINUM™ Functional Grade
Product No.: C6270
Clone DREG56 Target CD62L Formats AvailableView All Product Type Monoclonal Antibody Alternate Names TQ1, LAM1, LEU8, LNHR, LSEL, CD62L, LYAM1, PLNHR, LECAM1 Isotype Mouse IgG1 Applications B , FC , IF , IHC FF , in vivo , PhenoCycler® , WB |
Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829786 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this DREG56 antibody for staining cells in flow cytometry is ≤ 0.5 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this DREG56 antibody for use in western blotting is 1-10 μg/ml. Additional Applications Reported In Literature ? IHC FF IF B Additional Reported Applications For Relevant Conjugates ? PhenoCycler-Fusion (CODEX)® Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone DREG56 recognizes an epitope on human CD62L.
Background CD62L antibody, clone DREG56, recognizes CD62L, also known as L-selectin or LECAM-1, a 74-95 kDa type I transmembrane glycoprotein and cell adhesion molecule expressed on most circulating leukocytes1. Ligands for CD62L include CD34, GlyCAM-1, and MAdCAM-1, which require sialyation, fucosylation, and carbohydrate sulfation for recognition2,3. Ligand binding mediates tethering and rolling of leukocytes, facilitating entry into secondary lymphoid organs, including lymph nodes and Peyer’s patches, via high endothelial venules (HEVs)1. CD62L is also expressed on human embryo trophoblasts and has been proposed to play a role in human embryo implantation4.
Antigen Distribution CD62L is expressed on most circulating leukocytes, including neutrophils, monocytes, T, B and NK cells, and human embryo trophoblasts.
Ligand/Receptor CD34, GlyCAM, MAdCAM-1 Function Leukocyte homing, leukocyte tethering, rolling PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Costimulatory Molecules . Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone DREG56 is a monoclonal antibody that specifically targets human CD62L (L-selectin), and in in vivo mouse studies, it is primarily used for functional blocking or depletion of human CD62L, often in models involving human cells or humanized mice rather than native mouse CD62L.
In summary, in vivo mouse use of DREG56 is limited to humanized settings or xenografts, where it is employed to block or study human CD62L-dependent processes. For studies of mouse CD62L, other antibodies are required. Commonly Used Antibodies and Proteins with DREG56DREG56 is a monoclonal antibody clone targeting CD62L (L-selectin), widely used in immunology research, especially in studies involving leukocyte adhesion, trafficking, and immune cell phenotyping. While the search results focus on DREG56 itself, several standard antibodies and proteins are commonly used alongside DREG56 in the literature, though not specifically listed in these sources. Based on the broader context of CD62L biology and typical experimental setups, the following are often used with DREG56: Co-Staining Antibodies
Functional Assay Proteins
Negative Controls
Summary Table
These combinations are standard in immunology research focused on lymphocyte homing, adhesion, and migration, reflecting the central role of CD62L (detected by DREG56) in these processes. Always check for cross-reactivity and optimize panels based on specific experimental goals. Based on the scientific literature cited across multiple research applications, clone DREG56 has generated several key findings that have advanced our understanding of CD62L/L-selectin biology and immune function. Cellular Distribution and Expression PatternsThe DREG56 clone has been instrumental in characterizing the expression profile of CD62L across immune cell populations. Research has established that CD62L is expressed on neutrophils, monocytes, T- and B-lymphocyte subsets, and NK cells. The antibody recognizes a 74-95 kDa type I transmembrane glycoprotein that serves as a critical cell adhesion molecule on most circulating leukocytes. Lymphocyte Homing and Tissue SelectivityOne of the most significant discoveries using DREG56 is its role in defining lymphocyte homing mechanisms. The clone specifically inhibits over 90% of binding between human lymphocytes and high endothelial venules (HEV) in frozen sections of peripheral lymphoid tissue, but notably not in mucosal lymphoid tissue. This finding established L-selectin as a human lymphocyte homing receptor with tissue-specific functions, particularly for peripheral lymph node HEV targeting. Clinical Applications in Disease ResearchThe literature reveals extensive use of DREG56 in clinical research across multiple disease contexts: Cancer Research: Studies have utilized the clone to investigate immune profiles in cancer patients, with research examining cellular immune responses and potential therapeutic targets. Pediatric Medicine: Research has employed DREG56 to study immune development and function in pediatric populations, contributing to understanding age-related immune system variations. Infectious Disease Studies: The clone has been valuable in examining immune responses during infections, including detailed flow cytometric analysis of leukocyte populations during disease progression. COVID-19 Research ApplicationsRecent pandemic research has leveraged DREG56 for comprehensive immune profiling in COVID-19 patients. Studies have used high-dimensional flow cytometry with this clone to examine alterations in innate and adaptive immune compartments, revealing key myeloid signatures associated with severe disease. This work has contributed to understanding how immune cell characteristics and relative abundance change during COVID-19 progression. Technical Validation Across Multiple ApplicationsThe scientific literature demonstrates DREG56's versatility across various experimental techniques. Citations show successful applications in flow cytometry, western blotting, immunohistochemistry on frozen sections, and immunofluorescence. The clone has been validated for both research and clinical applications, with studies consistently demonstrating its reliability for detecting CD62L expression changes across different experimental conditions. These findings collectively establish DREG56 as a critical research tool that has significantly advanced our understanding of immune cell trafficking, homing mechanisms, and disease-associated immune alterations across diverse clinical and research contexts. There is no standardized in vivo dosing regimen for clone DREG56 (anti-human CD62L/L-selectin) across mouse models documented in the provided results. The available sources describe DREG56 primarily as a research-use-only (RUO) reagent for flow cytometry to detect human CD62L expression on human peripheral blood lymphocytes, not as a therapeutic or depleting antibody administered to living mice in preclinical models. Key Context and Clarifications:
Summary Table: DREG56 vs. Common Functional Antibodies in Mouse Models
If you seek in vivo dosing regimens for DREG56 in humanized mouse models or other contexts, this is not supported by the current literature or datasheets; DREG56 is not used for in vivo functional intervention in mice. Any proposed systemic use would require novel validation and justification. If you meant flow cytometry staining protocols or require guidance for other anti-CD62L clones used in mouse (cross-reactive) models, please clarify. References & Citations1. Ivetic A, et al. (2019) Front Immunol. 10:1068 2. Varki A (1994) Proc Natl Acad Sci USA. 91(16):7390-7397 3. Rosen SD. (1999) Am J Pathol. 155(4):1013-1020 4. Genbacev OD, et al. (1003) Science. 299(5605):405-8 Technical ProtocolsCertificate of Analysis |
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C6270 |
