Anti-Mouse CD80 [Clone 16-10A1] — Purified in vivo GOLD™ Functional Grade

Clone 16-10A1 is an anti-mouse CD80 (B7-1) monoclonal antibody widely used in in vivo mouse studies to block the costimulatory function of CD80, thereby modulating T cell activation and immune responses.

Key uses in in vivo mouse studies:

• Blockade of CD80-mediated costimulation: 16-10A1 prevents the interaction between CD80 and its receptors, CD28 (which stimulates T cells) and CTLA-4 (which inhibits T cells). This effectively blocks T cell activation signals mediated by CD80, allowing researchers to interrogate the role of CD80 in immunity, tolerance, and autoimmunity.
• Selective action: It specifically blocks CD80 without affecting the activity of CD86 (B7-2), the other main costimulatory molecule.
• In vivo applications: The unconjugated form of 16-10A1 is typically used for systemic administration in mice to block CD80 function in disease models, such as autoimmunity, infection, transplantation tolerance, and tumor immunity.
• Reported as In Vivo Ready/Low Endotoxin: Preparations marketed as "in vivo ready" or "low endotoxin" are specifically purified and quality-controlled for safe injection into mice.

Mechanistic details:

• CD80 Expression: Found on activated B cells, activated T cells, macrophages, and dendritic cells in mice.
• Administration: The antibody is typically injected intraperitoneally or intravenously at doses specified by experimental needs to achieve systemic blockade.

Other uses:

• While 16-10A1 is also used for flow cytometry and immunofluorescence in its conjugated forms, in in vivo studies the primary application is functional blockade, not detection.

Summary:
Clone 16-10A1 is used in vivo to experimentally block CD80-mediated T cell co-stimulation in mice, thus enabling investigation of CD80's immunological roles and its potential as a therapeutic target.

The correct storage temperature for the sterile packaged clone 16-10A1 (anti-CD80 monoclonal antibody) is 2–8°C (standard refrigerator temperature), protected from light, and it should not be frozen.

Most suppliers and technical datasheets specify this temperature range for both short-term and long-term storage to maintain product stability and functionality. Key guidance includes:

• Store at 2–8°C (refrigerator; typically equivalent to 4°C is acceptable within this range).
• Protect from prolonged exposure to light, as fluorochrome-conjugated antibodies (such as PE or FITC conjugates) are light-sensitive.
• Do not freeze; freezing can damage the antibody or affect its binding capability.

This guidance applies specifically to the sterile, ready-to-use, or undiluted antibody reagent version of clone 16-10A1, including common commercial formats. If the product is in a different formulation (e.g., lyophilized powder), additional instructions from the product datasheet should be followed.

The antibody 16-10A1 targets CD80 (B7-1), a co-stimulatory molecule involved in T cell activation. Commonly, antibodies or proteins used alongside 16-10A1 in the literature are those that modulate related immune pathways, particularly those associated with T cell signaling and co-stimulation.

Key antibodies and proteins commonly used with 16-10A1 include:

• Anti-CD86 (B7-2) antibodies: CD86 is the second major co-stimulatory ligand for CD28 and CTLA-4, often examined alongside CD80 for their complementary or redundant roles in T cell activation.
• CD28 blockade or recombinant CD28: Since CD80 and CD86 both interact with CD28, inhibitors or agonists for CD28 are frequently used in combination to dissect signaling pathways.
• CTLA-4 antibodies or CTLA-4-Ig fusion protein: Because CD80 binds CTLA-4 as well, blocking or modulating this interaction is common in immunological experiments focused on co-inhibitory signals.
• T cell surface markers (CD3, CD4, CD8): When studying T cell activation, antibodies targeting these core markers are routinely included to identify and sort T cell subpopulations.
• MHC class II antibodies: For functional antigen presentation studies that involve co-stimulation, antibodies blocking MHC class II are often used with 16-10A1.
• Other immune checkpoint molecules (PD-1, PD-L1): Frequently included in studies mapping immune regulatory networks in disease or therapy contexts.

Supporting context:

• In flow cytometry and functional blocking studies, anti-CD86 (clone GL1 or PO3) is typically paired with 16-10A1 to block both B7 ligands and assess their combined influence on T cell activation.
• In inhibition experiments, CTLA-4-Ig fusion proteins are used to block both CD80 and CD86 by outcompeting CD28 binding.
• Literature often includes combinations of 16-10A1 with antibodies for CD28 and CTLA-4 to parse out the contributions of each interaction to immune response outcomes.

These combinations are frequently observed in publications studying T cell costimulation, immune tolerance, transplant models, and cancer immunotherapy.

Clone 16-10A1 is a widely used monoclonal antibody targeting mouse CD80 (B7-1), primarily employed to study immune costimulation, T cell activation, and immune checkpoint biology in murine models. The key findings from citations of 16-10A1 in scientific literature are as follows:

• Specificity and Mechanism: 16-10A1 specifically binds to mouse CD80 and blocks its costimulatory function, preventing CD80 from interacting with its ligands CD28 (which stimulates T cells) and CTLA-4 (which inhibits T cells). Importantly, 16-10A1 does not block CD86, a closely related costimulatory molecule.

• Experimental Applications:

  • Used to block CD80 in vivo, allowing researchers to distinguish the unique roles of CD80 versus CD86 in immune responses such as T cell activation, autoimmunity, and tumor immunology.
  • Commonly applied in flow cytometry, in vivo functional blocking, and immunophenotyping protocols of murine tissues (e.g., spleen, lymph node, tumor microenvironment).

• Cellular and Tissue Distribution: CD80 is expressed on activated B cells, activated T cells, monocytes, macrophages, and dendritic cells. 16-10A1 is used to selectively stain and block CD80 on these populations.

• Cross-Species Use and Limitations: While 16-10A1 is essential for mouse studies, its cross-reactivity in other species (e.g., dog, rat, human) is variable and can lead to inconsistent results. For instance, in dogs, 16-10A1 has been reported to bind to neutrophils and monocytes in both healthy and leukemic (AML) cases—results that differ markedly from findings in mice and from other CD80 clones. This suggests potential cross-reactivity with other B7 family molecules or antigenic differences, prompting the need for caution and additional validation in non-murine species.

• Research Insights Uncovered by 16-10A1 Use:

  • Demonstrated the major role of CD80 in the costimulation and regulation of T cell responses, including in models of infection, tumor immunity, and autoimmunity.
  • Dissection of immune checkpoint pathways: The antibody is often used in conjunction with other inhibitors (such as anti-CD86 or anti-PD-L1) to parse out the individual contributions of costimulatory/inhibitory signals in vivo.
  • Revealed differences in antigen detection depending on the clone used, sample type, species, and technical conditions—emphasizing the importance of proper controls and antibody validation in immunological experiments.

• Notable Citation Trends: Clone 16-10A1 is routinely cited in foundational and applied studies on the immunobiology of T cell responses, checkpoint blockade, and immune regulation in murine models, particularly in cancer immunotherapy and transplantation literature.

In summary, clone 16-10A1 is a gold standard reagent for blocking and detecting murine CD80, critical for dissecting T cell costimulation and checkpoint regulation, but its use outside standard murine systems requires rigorous validation due to potential cross-reactivity and species differences.