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Antibody DetailsProduct DetailsReactivity Species Mouse Host Species Armenian Hamster Immunogen Purified Recombinant Mouse CD80 (>98%) Product Concentration ≥ 5.0 mg/ml Endotoxin Level ≤ 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2749825 Other Applications Reported In Literature ? CyTOF® Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone 16-10A1 recognizes an epitope on mouse CD80. Antigen Distribution CD80 is expressed on activated B cells, monocytes/macrophages, and dendritic cells. Background CD80 is a highly glycosylated 60 kD protein that is part of the Ig superfamily and is significantly involved in immune cell activation in response to pathogens. CD80 is closely related to, and works in tandem with CD86 (B7-2) to prime T- cells. CD80 binds to CTLA-4 to deliver an inhibitory signal to T cells. The ligation of CD28 on T cells with CD80 and CD86 on APCs co-stimulates T cells resulting in enhanced cell activation, proliferation, and cytokine production. It is thought that CD80 interacts with a ligand on Natural Killer cells, activating the Natural Killer cell-mediated cell death of the CD80 carrier. The activation of Natural Killer cell-mediated death via CD80 interactions has potential as a possible cancer immunotherapy through the induction of CD80 expression on tumor cells. Antigen DetailsProtein Ligand/Receptor CD28 (stimulatory), CD152(CTLA4) (inhibitory) Function T cell costimulation PubMed NCBI Gene Bank ID UniProt.org Research Area Cell Biology . Costimulatory Molecules . Immunology . Neuroscience . Neuroscience Cell Markers References & Citations1.) Gubin, M. et al. (2018) Cell. 175(4):1014–1030.e19 Journal Link Technical Protocols  |