Anti-Mouse CD172a [Clone P84] – Alexa Fluor® 647

Mouse brain membrane protein

This Alexa Fluor® 647conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Alexa Fluor® is a trademark of Life Technologies Corporation.

Liquid

This Alexa Fluor® 647 conjugate is stable when stored at 2-8°C. Do not freeze.

Research Use Only

2-8°C Wet Ice

Red Laser (633 nm) Alexa Fluor® 647 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.

Clone P84 recognizes an epitope on mouse CD172a.

CD172a antibody, clone P84, recognizes CD172a, also known as single regulatory protein α (SIRPα) (signal regulatory protein alpha) or Src homology 2 domain-containing phosphatase substrate-1 (SHP-1), a type I transmembrane glycoprotein with three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibition motifs (ITIMs)¹. SIRPα is expressed predominantly in myeloid cells² - including monocytes, macrophages, and dendritic cells (DCs) - and neuronal cells³. The extracellular ligand for SIRPα, CD47 (or integrin-associated protein [IAP])⁴, is expressed in most cell types⁵. In macrophages, ligation of SIRPα by CD47 inhibits macrophage phagocytosis of self cells^6,7. SIRPα also negatively regulates DC-mediated T cell activation and DC maturation^8-10. CD47 is also upregulated on tumor cells, inhibiting the phagocytosis of tumor cells by macrophages¹¹. Therapeutics targeting the CD47-SIRPα interaction, including antibodies and fusion proteins, are currently under preclinical and clinical study for various malignancies as a monotherapy or in combination with other therapeutics¹².

CD172a is expressed on monocytes, macrophages, dendritic cells, and neuronal cells.

CD47, SP-A, SP-D

Negative regulation of several biological processes

1. Fujioka, Y., et al. (1996) Mol. Cell. Biol. 16:6887
2. Adams, S., et al. (1998) J. Immunol. 161:1853
3. Chuang W, et al. (1990) Dev Biol. 137:219–232
4. Seiffert, M., et al. (1999) Blood 94:3633
5. Oldenborg P. A. (2013) ISRN Hematol. 2013:614619
6. Oldenborg, P. A., et al. (2000) Science 288:2051
7. Oldenborg, P. A., et al. (2001) J. Exp. Med. 193:855
8. Brooke, G. P., et al. (1998) Eur. J. Immunol. 28:1
9. Seiffert, M., et al. (2001) Blood 97:2741
10. Latour, S., et al. (2001) J. Immunol. 167:2547
11. Jaiswal S, et al. (2009) Cell. 138(2):271-85
12. Jalil AR, et al. (2020) Antib Ther. 3(2):80-94