Anti-Mouse/Human IL-5 – Purified in vivo GOLD™ Functional Grade
Anti-Mouse/Human IL-5 – Purified in vivo GOLD™ Functional Grade
Product No.: I-1060
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Clone TRFK5 Target IL-5 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names BCDFII,Interleukin-5, IL5, B cell differentiation factor 1, TRF, EDF Isotype IgG1 Applications CyTOF® , Depletion , ELISA Cap , ELISPOT , FA , FC , IHC FFPE , in vivo , N , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Partially-purified T cell clone supernatant Product Concentration ≥ 2.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling This Low Endotoxin Functional Formulation, GOLD Antibody is stable when stored at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2830393 Applications and Recommended Usage? Quality Tested by Leinco FC: The suggested concentration for this TRFK5 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. NOTE: A fluorochrome-conjugated TRFK51 antibody would be useful for intracellular staining and flow cytometric analysis to identify IL-5-producing cells within mixed cell populations. ELISA:This purified TRFK5 antibody is useful as a capture antibody for a sandwich ELISA assay. A suitable detection antibody would be a biotinylated clone TRFK4 antibody for mouse IL-5, or used in conjunction with biotinylated JES1-5A10 antibody. Additional Applications Reported In Literature ? NeutralizationThis Purified In vivo GOLD™ Functional Grade antibody (Endotoxin <1.0 EU/mg) is recommended for neutralization of human IL-5 bioactivity.
CyTOF® ELISA Cap FA IHC (Paraffin) WB Depletion Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone TRFK5 recognizes mouse/human IL-5. Antigen Distribution Mast cells, T cells, eosinophils Background IL-5 is a 26 kD TH2 cytokine and a homodimeric glycoprotein that is part of the hematopoietic family. IL-5 stimulates B cell growth as well as increases Ig secretion. It is the main factor that promotes the terminal differentiation of eosinophil progenitors and enhances the effector capacity of mature eosinophils. IL-5 is a major regulator of eosinophil accumulation in tissues. In fact, in Hodgkin lymphoma, the characteristically observed eosinophilia is suspected to result from an increased production of IL-5. IL-5 may also be the cause of several allergic diseases including asthma, where it is thought that eosinophils have a significant role in the disease pathology. There are several mAbs currently in use that target IL-5 for the treatment of severe eosinophilic asthma. Antigen DetailsFunction Growth/differentiation of B cells, eosinophils; proliferation/cell activation/differentiation of eosinophilic granulocytes PubMed NCBI Gene Bank ID UniProt.org Technical ProtocolsCyTOF® Depletion  ELISPOT FA  IHC FFPE  N  |
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Prod No. | Description |
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I-1038 | |
I-1063 | |
I-1064 | |
I-1062 | |
I-1060 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
