Anti-Mouse IFNγ (Clone XMG1.2) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.I-1209
Clone
XMG1.2
Protein
IFN-γ
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Immune Interferon, Type II Interferon, T Cell Interferon, MAF, IFNG, IFG, IFI
Isotype
Rat
IgG1 κ
Applications
CyTOF®
,
ELISA Cap
,
ELISPOT
,
ICFC
,
IHC FF
,
N
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-1209-1.0 mg
1.0 mg
$198.00
In stock
Max:
Min: 1
Step: 1
I-1209-5.0 mg
5.0 mg
$398.00
In stock
Max:
Min: 1
Step: 1
I-1209-25 mg
25 mg
$1,150.00
In stock
Max:
Min: 1
Step: 1
I-1209-50 mg
50 mg
$1,825.00
In stock
Max:
Min: 1
Step: 1
I-1209-100 mg
100 mg
$2,500.00
In stock
Max:
Min: 1
Step: 1

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Purified Recombinant Mouse IFN-γ (>98%)
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUMTM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Cap XMG1.2 antibody is useful as a detection antibody for a sandwich ELISA with a concentration range of 0.5-2.0 µg/ml as recommended.
WB The suggested concentration for this XMG1.2 antibody for use in western blotting is 1-10 μg/ml.
Other Applications Reported In Literature ?
ICFC
ELISPOT Cap
ELISPOT Det
CyTOF®
N
Additional Reported Applications For Relevant Conjugates ?
IHC FF
WB

For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rat Anti-Mouse Interferon Gamma (IFN-γ) (Clone XMG1.2) recognizes an epitope on Mouse IFN-γ. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
Interferon-gamma (IFN-γ) or type II interferon is a dimerized soluble cytokine that is the only member of the type II class of interferons.1 It is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. IFNG is produced predominantly by natural killer (NK) and natural killer T (NKT) cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte (CTL) effector T cells once antigen-specific immunity develops.2 IFN-γ has antiviral, immunoregulatory, and anti-tumour properties.3

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
.
Immunology
.
Neuroinflammation
.
Neuroscience

References & Citations

1.) Goeddel DV et al. (1982) Nature 298: 859
2.) Wilson CB et al. (2007) Adv. Immunol. 96: 41
3.) Hume DA et al. (2004) J Leukoc Biol. 75: 163
4.) Teijaro et al. (2020) bioRxiv 22: 24
5.) Hawman DW, et al. (2021) Microorganisms 9(2):279 Journal Link
Elisa Sandwich Protocol
IHC
General Western Blot Protocol