Anti-Mouse NK1.1 [Clone PK136] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse NK1.1 [Clone PK136] — Purified in vivo GOLD™ Functional Grade
Product No.: N123
Clone PK136 Target NK1.1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Natural Killer Cells, KR-P1C, NKR-P1B, Ly-55, CD161b, CD161c Isotype Mouse IgG2a k Applications B , CyTOF® , Depletion , FC , in vivo , IP , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen NK-1+ cells from mouse spleen and bone marrow Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal NK1.1 antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737553 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: The suggested use of this NK1.1 antibody is ≤ 0.25 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application. Additional Applications Reported In Literature ? CyTOF® (Validated) IP Depletion Block IHC IF (Reported in the literature) Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This NK1.1 antibody (clone PK136) recognizes mouse NK1.1. Background NK1.1 is a type II membrane protein that is part of the C-type lectin superfamily. NK1.1 contains a transmembrane domain and a cytoplasmic domain that are characteristic of C-type lectins. The function of NK cells is to mediate cytotoxicity and to secrete cytokines after immune stimulation. NK1.1 has been correlated with lysis of tumor cells In vitro and rejection of bone marrow allografts In vivo. It is also involved in NK cell activation, IFN-γ production, and cytotoxic granule release. NK-1.1 is commonly used as a mouse NK cell marker. Antigen Distribution NK-1.1 is encoded by the NKR-P1B/NKR-P1C gene and expressed on NK cells and NK-T cells in some mouse strains, including C57BL/6, FVB/N, and NZB, but not AKR, BALB/c, CBA/J, C3H, DBA/1, DBA/2, NOD, SJL, and 129. Function NK cell activation, IFN-γ production, cytotoxic granule release PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone PK136 is most commonly used in vivo in mice for the specific depletion (removal) of natural killer (NK) cells in strains expressing the NK1.1 (CD161, NKR-P1C) antigen, such as C57BL/6, FVB/N, NZB, and CE. Essential context and supporting details:
Common Strains and Specificity:
Experimental Considerations:
Summary Table: Common In Vivo Applications of Clone PK136 in Mice
Key references emphasize that NK cell depletion is the most reliable and frequent in vivo application in experimental immunology using mouse models. The most commonly used antibodies or proteins alongside PK136 in the literature are anti-CD3e, anti-CD49b (DX5), and anti-Asialo GM1. These are frequently employed in NK cell identification, functional studies, and depletion experiments using mouse models. Key antibodies and proteins often used with PK136:
Other reagents sometimes seen with PK136:
Summary of usage:
These combinations enable robust identification, depletion, and study of NK cells in mouse immunology research. The precise selection depends on the experimental design, mouse strain, and whether the goal is identification, depletion, or functional modulation. Clone PK136 is a mouse monoclonal antibody that binds specifically to NK1.1, a cell surface antigen expressed predominantly on mouse natural killer (NK) cells and a subset of T cells in certain mouse strains. The major scientific findings from literature that cite clone PK136 can be summarized as follows:
In science, clone PK136 is recognized for its specificity, efficiency, and utility in marking or depleting NK cells, which has significantly advanced the study of immune responses in mice. However, careful consideration of strain specificity, dosing, and potential off-target effects is required for experimental design. Dosing regimens of the anti-NK1.1 antibody clone PK136 in mouse models typically range from 200 to 500 μg per mouse per dose, most often delivered by intraperitoneal injection, with schedules and doses varying according to mouse strain, experimental goal, and desired depletion duration. Key dosing regimens for PK136 include:
Factors influencing dosing variation:
Supporting details:
In summary, while 200–300 μg/mouse i.p., 1–3 times per week is the most widely used regimen, optimal dosing depends on strain, age, experimental design, and depletion duration, with some models requiring initial higher doses or more frequent administration for complete NK or NKT cell removal. References & Citations1.) Ardolino, M. et al. (2018) J Clin Invest. 128(10):4654-4668. PubMed 2.) Koo, GC. and Peppard, JR. (1986) Hybridoma 3:301 3.) Raju et al. (2019) Cell Reports. 29:2556–2564 Journal Link Technical ProtocolsCertificate of Analysis |
Related Products
Prod No. | Description |
---|---|
S211 | |
R1364 | |
I-118 | |
M1188 | |
C247 | |
F1175 | |
S571 |
Formats Available
Prod No. | Description |
---|---|
N228 | |
N106 | |
N109 | |
N108 | |
N107 | |
N110 | |
N220 | |
N221 | |
N222 | |
N224 | |
N226 | |
N123 | |
N268 |
