Goat Anti-Mouse IgG (H&L) — Biotin

Purified Mouse IgG (H&L) (>98%)

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Biotin conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.

One year from date of reconstitution

Next Day Ambient

The suggested working dilutions for the following applications are:

ELISA: 1:20,000 – 1:400,000.

WB: 1:20,000 – 1:400,000.

IHC: 1:500 – 1:5000.

FC: 1:200 – 1:1,000.

IF: 1:200 – 1:1,000.

NOTE: Titration of the reagent is recommended for optimal performance for each application.
Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Each investigator should determine their own optimal working dilution for specific applications.

Goat Whole Molecule Anti-Mouse IgG (H&L) recognizes Mouse IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.