Anti-Human CD4 [Clone OKT-4] — Purified
Anti-Human CD4 [Clone OKT-4] — Purified
Product No.: C9210
Data
Western Blot
A 0.5ug sample of Human CD4 (Leinco Prod. No.: C9210) was loaded onto an SDS-PAGE gel under reducing conditions and probed with OKT-4 @ 10ug/mL, Goat Anti-Mouse IgG secondary antibody 1:1000 dilution (Goat Anti-Mouse IgG (H+L) adsorbed against ms HRP Leinco Prod. No.: M114)
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Human peripheral T cells Product Concentration 0.5 mg/ml Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This purified antibody is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829881 Applications and Recommended Usage? Quality Tested by Leinco ELISA FC WB Additional Applications Reported In Literature ? IF Staining IP IHC FF ICC N Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone OKT4 recognizes Human CD4. This clone recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not interfere with each other. Background CD4 is a 55 kD single-chain type I transmembrane glycoprotein and a member of the Ig superfamily. It is involved in the recognition of MHC class II molecules. Furthermore, CD4 is the primary receptor for HIV. CD4 initiates the early phase of T-cell activation via its association with the T-cell receptor complex and protein tyrosine kinase, Lck. In addition, CD4 functions as a mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Antigen Distribution CD4 is expressed on most thymocytes, a subset of T cells, and monocytes/macrophages, and may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology References & Citations1. Crotty, S. et al. (2019) Cell Rep 29(7):1756-66. 2. Ploss, A. et al. (2018) Nat Commun 9(1):5031 3. Lone, YC. et al. (2017) PLoS One.12(4): e0173754. Technical Protocols  ICC IF Staining IHC FF  N  Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
