Anti-Human CD62L – Purified in vivo GOLD™ Functional Grade

Clone DREG56 is a mouse monoclonal antibody that specifically binds human CD62L (L-selectin), a cell adhesion molecule expressed on neutrophils, monocytes, and subsets of T, B, and NK cells. In in vivo mouse studies, DREG56 is primarily used in xenograft or humanized mouse models, not in standard (wild-type) mice, because it is specific to human CD62L and does not cross-react with murine CD62L.

DREG56 is used for:

• Blocking or depleting human CD62L-positive leukocytes in humanized mice (mice engrafted with human immune cells), allowing researchers to dissect the roles of human CD62L in lymphocyte homing, trafficking, or immune response.
• Labeling or tracking human CD62L-positive cells by flow cytometry or immunofluorescence in tissues from humanized or xenografted mice, enabling quantification and localization of human leukocyte subsets during inflammation, tumor infiltration, or infection.
• Functional studies, such as inhibition of lymphocyte trafficking to lymph nodes via high endothelial venules (HEV), since DREG56 blocks >90% of human lymphocyte binding to HEV in vitro and in tissue sections.

Key limitations and context:

• DREG56 is not suitable for studying endogenous mouse (murine) CD62L due to its strict human specificity.
• Its use in vivo is thus generally limited to mice reconstituted with human immune cells (e.g., humanized MISTRG or NSG mice) to study human cell function, trafficking, or to deplete/block specific human leukocyte subsets.

In summary, DREG56 is essential in vivo for functional, blocking, or depletion studies of human CD62L-positive cells in humanized mouse models, and for tracking or phenotyping human leukocytes in those settings.

The correct storage temperature for sterile packaged clone DREG56 antibody is 2°C to 8°C (refrigerator temperature), and it should be protected from prolonged exposure to light and must not be frozen.

• Most manufacturers specify storage undiluted between 2°C and 8°C for clone DREG56 antibodies.
• Some sources specifically mention storing at 4°C, which falls within the recommended range.
• All emphasize do not freeze and advise protecting from prolonged light exposure to preserve antibody stability.

This storage guidance is consistent across multiple suppliers and applies to both purified and conjugated forms of the antibody.

Other commonly used antibodies or proteins studied alongside DREG56 (anti-CD62L) in the literature include those targeting CD44, as well as antibodies against markers like CD3, CD4, CD8 (T-cell markers), CD19 (B-cell marker), and CD45 (pan-leukocyte marker).

Relevant context and supporting details:

• CD44: CD44 is frequently mentioned as a functionally related molecule to CD62L, particularly in studies on cell adhesion, migration, and immune cell trafficking. Both play roles in lymphocyte homing and inflammation, and are often analyzed together to characterize leukocyte subpopulations and function.
• Other cell surface markers: In flow cytometry experiments, it is common to combine DREG56 with antibodies against CD3, CD4, CD8, CD19, and CD45 to phenotype lymphocytes, distinguish naive vs. memory T cells, or identify specific immune cell subsets.
• Functional studies: Antibodies to CD62L (DREG56) are frequently used in blocking experiments to study lymphocyte adhesion to high endothelial venules (HEV), often with comparison to blocking antibodies for CD44 and other selectins (such as E-selectin and P-selectin), to delineate the specific contributions of these molecules.
• Example protocols and publications: Citations in antibody datasheets and product literature refer to studies where DREG56 is used in parallel with antibodies against other homing and activation markers, measuring changes in surface phenotype or functional interactions in immunological research.

Summary of common combinations with DREG56:

• CD44
• CD3, CD4, CD8 (T cells)
• CD19 (B cells)
• CD45 (pan-leukocyte)
• E-selectin (CD62E), P-selectin (CD62P) (other selectin family members)

This approach enables detailed characterization of immune cell migration, homing, and subset differentiation by analyzing multiple relevant adhesion and activation molecules in parallel.

The clone DREG56, which recognizes the CD62L antigen (L-selectin/LECAM-1), has generated significant findings across multiple areas of immunological research based on its citations in scientific literature.

Functional Characterization and Binding Properties

DREG56 has been instrumental in defining the functional properties of L-selectin. The antibody specifically inhibits more than 90% of binding of human lymphocytes to high endothelial venules (HEV) in frozen sections of peripheral lymphoid tissue, but notably not in mucosal lymphoid tissue. This finding established L-selectin as a critical human lymphocyte homing receptor specifically for peripheral lymph node HEV, demonstrating tissue-specific adhesion mechanisms.

Expression Patterns and Cell Distribution

Research using DREG56 has mapped the comprehensive expression pattern of CD62L across immune cell populations. The antibody has confirmed that CD62L is expressed on neutrophils, monocytes, T- and B-lymphocyte subsets, and NK cells. Flow cytometric studies have provided detailed characterization of CD62L expression levels on human peripheral blood lymphocytes, contributing to our understanding of immune cell phenotypes.

Clinical and Translational Applications

Several clinical studies have utilized DREG56 to investigate disease mechanisms and therapeutic targets. Research has examined CD62L expression in cancer contexts, with studies published in Clinical Cancer Research exploring its role in tumor immunology. Additionally, pediatric research has investigated CD62L expression patterns in neonatal and developmental contexts, as documented in Pediatric Research publications.

Immunological Function Studies

The antibody has been extensively used to study neutrophil and immune cell trafficking. Publications in The Journal of Immunology have employed DREG56 to investigate various aspects of immune cell migration and function, including studies on neutrophil behavior and immune cell interactions. These studies have contributed to understanding how L-selectin mediates initial adhesion events in the inflammatory cascade.

Advanced Research Applications

Recent citations demonstrate DREG56's utility in cutting-edge research methodologies, including single-cell analysis approaches like CITE-seq and REAP-seq through TotalSeq™ conjugates. This has enabled simultaneous analysis of CD62L protein expression alongside mRNA profiles in individual cells, advancing our understanding of immune cell heterogeneity and function at unprecedented resolution.

The breadth of citations spanning from basic immunology research in PNAS to clinical applications demonstrates DREG56's value as a research tool for understanding L-selectin biology across multiple biological systems and disease contexts.