Anti-Human HLA-A, B, C (MHC Class I) [Clone W6/32] — Purified in vivo GOLD™ Functional Grade
Anti-Human HLA-A, B, C (MHC Class I) [Clone W6/32] — Purified in vivo GOLD™ Functional Grade
Product No.: H263
Clone W6/32 Target HLA-A,B,C Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Major Histocompatibility Class I, MHC class I, human leukocyte antigen (HLA) Isotype Mouse IgG2a k Applications B , FC , IHC FF , in vivo , IP , PhenoCycler® , WB |
Antibody DetailsProduct DetailsReactive Species Baboon ⋅ Chimpanzee ⋅ Cynomolgus Monkey ⋅ Feline ⋅ Bovine ⋅ Human Host Species Mouse Recommended Isotype Controls Recommended Isotype Controls Recommended Dilution Buffer Immunogen Human tonsil cell membrane Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2737521 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this HLA-A,B,C Clone W6/32 antibody for staining cells in flow cytometry is ≤ 2.0 μg per 106 cells in a volume of 100 μl or 100μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for for use in western blotting is 1-10 μg/ml. Additional Applications Reported In Literature ? B CODEX® IHC FF IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone W6/32 recognizes the human MHC class I molecules HLA-A, -B, and -C. Background HLA antibody, clone W6/32, recognizes the major histocompatibility complex (MHC) class I molecules human leukocyte antigen (HLA)-A, HLA-B, and HLA-C. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. MHC class I plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11. Antigen Distribution HLA-A, -B, and -C are ubiquitously expressed on nucleated cells.
Ligand/Receptor CD3/TCR, CD8 Function Antigen presentation PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology . Innate Immunity Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. W6/32 is a mouse monoclonal antibody that specifically recognizes a monomorphic epitope on human HLA class I molecules (i.e., HLA-A, HLA-B, HLA-C), and is widely used in human immunology for detection of these antigens. In in vivo mouse studies, its direct use is very limited due to species specificity; the W6/32 antibody reacts weakly or not at all with native mouse MHC class I molecules unless specific experimental manipulations are performed. Key context and limitations in mouse studies:
Applications and practical caveats in mouse models:
Summary Table: W6/32 Use in Mice
In summary, W6/32 is not used for general MHC class I detection in wild-type mice. It can only be used to detect HLA class I in genetically modified or xenografted mice, or in murine cells experimentally complexed with human or bovine ?2-microglobulin. The correct storage temperature for sterile-packaged clone W6/32 (anti-human HLA-A,B,C antibody) is 2°C to 8°C (refrigerated), and the antibody should be kept undiluted and protected from prolonged exposure to light. Do not freeze this antibody, as freezing can denature or reduce its effectiveness.
If your sample is specifically an Ultra-LEAF or similar preservative-free variant, maintain strict aseptic conditions and keep at 2–8°C. Summary table:
Maintain the antibody undiluted and protected from light during storage for optimal stability. Commonly used antibodies or proteins often paired with W6/32 in the literature include:
In sum, W6/32 is commonly combined with anti-?2-microglobulin, allele-specific anti-HLA antibodies, class II antibodies, lineage markers, isotype controls, and other conformational antibodies to HLA class I to dissect surface expression, molecular assembly, or cell subset specificity in immunology and transplantation literature. The monoclonal antibody W6/32 is a key reagent in immunology, widely cited for its role in detecting human HLA class I molecules (HLA-A, HLA-B, HLA-C). Key findings from its use in scientific literature include:
In summary, W6/32s primary scientific importance lies in enabling highly sensitive, specific, and reproducible detection, quantification, and purification of native HLA class I molecules, underpinning foundational studies in immunogenetics and antigen presentation. References & Citations1. Mitaksov V & Fremont DH. (2006) J Biol Chem. 281(15):10618-25 2. Wieczorek M, et al. (2017) Front Immunol. 8:292 3. Jones EY. (1997) Curr Opin Immunol. 9(1):75-9 4. Matsumura M, et al. (1992) Science. 257:927–34.10.1126/science.1323878 5. Bouvier M & Wiley DC. (1994) Science. 265:398–402.10.1126/science.8023162 6. Zacharias M & Springer S. (2004) Biophys J. 87:2203–14.10.1529/biophysj.104.044743 7. Cruz FM, et al (2017) Annu Rev Immunol. 35:149-176 8. Artyomov MN, et al (2010) Proc Natl Acad Sci USA. 107(39):16916-16921 9. Orr MT & Lanier LL. (2010) Cell. 142(6):847-856 10. Raulet DH. (1994) Adv Immunol. 55:381-421 11. Salcedo M & Ljunggren HG. (1996) Chem Immunol. 64:44-58 Technical ProtocolsCertificate of Analysis |
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