Anti-Human HLA-DQ (MHC Class II) [Clone 1a3] — Purified in vivo GOLD™ Functional Grade

The clone 1a3 is not explicitly mentioned in the context of in vivo mouse studies in the provided search results. However, clone 1a3 is known to react with human HLA-DQ and is used in Western blotting applications, reacting with virtually all B-cell lines and some tissue macrophages and dendritic cells. It does not react with HLA-DR.

Another unrelated study mentions the use of a reprogramming cocktail denoted as 1a3 in the context of converting mouse fibroblasts into induced hepatic stem cells (iHepSCs), but this is not related to the clone 1a3 used for reacting with HLA-DQ.

For specific details on how clone 1a3 is used in in vivo mouse studies related to HLA-DQ, additional information would be needed.

The correct storage temperature for a sterile packaged monoclonal antibody such as clone 1a3 is typically between 2°C and 8°C (refrigerated conditions) for short-term storage, according to widely accepted laboratory standards for monoclonal antibodies.

For short-term storage (up to several weeks), keep the antibody at 2–8°C (in a refrigerator). For long-term storage (several months or longer), it is best stored at -20°C or -80°C to maximize stability and prevent degradation.

Key details:

• Avoid repeated freeze-thaw cycles by aliquoting the product as needed.
• Store in the back of the refrigerator or freezer to minimize temperature fluctuations.
• For very long-term or critical preservation, store at -80°C if feasible.

If the sterile packaging refers to sterilized items in general (not antibodies), general standards for sterile medical item storage recommend keeping the temperature between 18°C and 23°C with 30–60% relative humidity to maintain packaging integrity.

If you have a specific manufacturer's datasheet for clone 1a3, always follow those instructions, as some products may have unique requirements.

Summary: For most sterile packaged monoclonal antibodies, store refrigerated at 2–8°C unless the manufacturer specifies otherwise. For general sterile items, store at 18–23°C.

Several commonly used antibodies and proteins are frequently used alongside the 1a3 antibody (typically referring to anti-Rap1A, clone 1D4-1A3) in the literature, depending on the experimental context and application.

• In immunocytochemistry, immunofluorescence, immunohistochemistry, immunoprecipitation, and Western blot studies with 1a3, researchers often use antibodies against related proteins in the Ras superfamily (such as Rap1B, Ras, or other GTPases), housekeeping proteins (such as ?-actin, GAPDH, or tubulin for loading controls), and signaling pathway components (such as ERK, MEK, or GEFs/GAPs relevant to Rap1A signaling).

• Proximity Ligation Assay (PLA) studies using 1a3 have paired it with antibodies against proteins that interact with Rap1A to visualize protein-protein interactions.

• Additionally, alternative or complementary binding reagents to antibodies are described in the literature, including monobodies, DARPins, affibodies, anticalins, and affimers. While not always used "with" 1a3 specifically, these engineered proteins are gaining traction as antibody alternatives or adjuncts for specific detection of protein targets in imaging, immunoprecipitation, or other biotechnological assays.

• In certain immunogenetic assays, HLA class I and II proteins or synthetic peptides are used with antibodies to study binding epitopes or immune responses. These combinations are highly context-specific but illustrate the common practice of pairing antibodies with peptides or recombinant proteins to dissect protein interactions or immune recognition events.

Summary of commonly used antibodies and proteins with 1a3:

• Other Ras-family or GTPase antibodies (e.g., anti-Rap1B, anti-Rap2)
• Housekeeping protein antibodies for controls (e.g., anti-?-actin)
• Signaling pathway markers (e.g., anti-ERK, anti-MEK)
• PLA partner antibodies when visualizing interactions
• Synthetic peptides or recombinant proteins for binding studies
• Alternative binding reagents: monobodies, DARPins, affibodies, anticalins

These combinations enable multiplexing, validation, and broader mechanistic studies in cell biology, immunology, and protein biochemistry research.

I cannot provide comprehensive key findings for clone 1A3 based on the available search results, as they contain limited and fragmented information about this specific clone.

From the search results provided, I found only two brief mentions of clone 1A3, each in different research contexts:

Nanopen Technology for Antibody Discovery

In one study focused on single B cell antibody discovery, clone 1A3 was associated with nanopen 928, which demonstrated changes in intensity and size during a screening process over a 45-minute timepoint. This appears to be part of research on rapid antibody discovery techniques using nanotechnology platforms.

Myomaker Function in Cell Fusion

In muscle biology research, clone 1A3 was identified as one of three myomaker knockout (KO) clones (along with 1B1 and 1B5) that could differentiate into muscle cells, as evidenced by myosin staining, but failed to undergo cell fusion. This suggests that myomaker protein is essential for the fusion process in muscle cell development.

The search results do not provide sufficient detail about clone 1A3's specific characteristics, experimental outcomes, or broader scientific significance. To obtain comprehensive findings about clone 1A3 citations in scientific literature, additional search results containing more complete research articles and citations would be necessary.