Anti-Human HLA-DQ (MHC Class II) [Clone 1a3] — Purified in vivo PLATINUM™ Functional Grade

Clone 1a3 has been used in in vivo mouse studies as a transcription factor cocktail—specifically, a combination of Hnf1? and Foxa3—to directly reprogram mouse fibroblasts into induced hepatic stem cells (iHepSCs) and subsequently into induced cholangiocyte progenitor cells (iCPCs). This approach enables the study of liver regeneration and diseases involving cholangiocyte dysfunction in murine models.

Key points on the in vivo use:

• 1a3-transduced mouse embryonic fibroblasts (MEFs) exhibit mature hepatic stem cell and cholangiocyte marker expression, yielding robust iHepSC colonies.
• In vivo testing: 1a3-iCPCs were transplanted into mice fed with 3,5-diethoxycarbonyl-1,4-dihydrocollidine (DDC), a chemical that induces bile duct injury, to test their regeneration and differentiation capacity in the diseased mouse liver.
• Findings: The transplanted 1a3-iCPCs contributed to bile duct proliferation and regeneration, modeling repair of cholangiocyte-related liver injury in vivo.
• The 1a3-mediated conversion closely mimics the natural regenerative pathways observed during rapid liver regeneration after partial hepatectomy, providing a physiologically relevant model for liver disease and regenerative studies.

If you intended "clone 1a3" to refer to the antibody clone 1A3 (e.g., anti-Alix/Xp95 antibody), it is primarily used for immunocytochemistry, immunoprecipitation, and western blot in cell and tissue samples, with reactivity toward human and predicted reactivity in mouse based on sequence homology, but in vivo mouse usage is not clearly specified in the provided references.

In summary, clone 1a3 in the context of direct reprogramming is used to generate hepatic stem/progenitor cells, which are then tested in mouse models of liver injury for studying regeneration and disease mechanisms. If you were referring to an antibody, please clarify further, as its in vivo use in mouse studies remains less clearly documented in available sources.

Based on the available information, I cannot determine the specific correct storage temperature for the sterile packaged clone 1A3 without additional product-specific documentation or specifications.

General Storage Temperature Guidelines

For sterile packaged biological materials and pharmaceutical products, standard storage temperatures typically follow established guidelines:

Standard Temperature Categories:

• Freezer Storage: -25°C to -10°C
• Refrigerator Storage: +2°C to +8°C
• Cool Storage: +8°C to +15°C
• Controlled Room Temperature: +20°C to +25°C

For general sterile storage conditions, maintaining a temperature between 18°C and 23°C with relative humidity between 30% and 60% is recommended, with items stored in enclosed storage units to prevent contamination.

Cell Line Storage Considerations

For cell lines similar to the Jurkat-VAV1-HiBiT-KI clone mentioned in the search results, cryogenic storage protocols typically involve freezing cells overnight in a -80°C freezer before transferring to liquid nitrogen storage. However, this applies to long-term cryopreservation rather than short-term sterile storage.

Pharmaceutical Storage Standards

The USP <659> guidelines have introduced a "Controlled Cold" category (+2°C to +15°C) that allows for some temperature flexibility while maintaining product integrity, with mean kinetic temperature not exceeding +8°C.

Recommendation: You should consult the specific product documentation, manufacturer's instructions, or certificate of analysis for clone 1A3 to determine the exact storage temperature requirements, as different biological products may have unique storage needs based on their composition and intended use.

Antibodies Commonly Used with 1a3 in the Literature

The term "1a3" appears in at least one context as a clone identifier for an anti-HLA-DQ (MHC class II) antibody. However, there is no direct evidence in the provided search results that 1a3 is widely referenced alongside specific, commonly used companion antibodies or proteins in the published literature.

HLA-DQ (MHC Class II) Research Context

HLA-DQ is a major histocompatibility complex (MHC) class II molecule, and antibodies like clone 1a3 are used to detect or study this protein in immunological research, particularly in transplantation and autoimmune studies. While the search results do not list other antibodies routinely paired with 1a3, related research in this field often utilizes:

• Anti-HLA-DR antibodies: HLA-DR is another MHC class II molecule frequently studied in parallel with HLA-DQ in immunology.
• Anti-CD4 antibodies: CD4+ T cells interact with MHC class II molecules, so CD4 detection is common in studies involving HLA-DQ.
• Anti-CD19 or anti-CD20 antibodies: These are used to identify B cells, which can present antigen via MHC class II.
• Secondary antibodies conjugated to fluorophores or enzymes: For flow cytometry (e.g., APC as in the commercial 1a3 product) or immunohistochemistry.

However, none of these are explicitly cited as being used alongside clone 1a3 in the provided literature.

Broader Protein and Antibody Alternatives

In the field of protein detection and imaging, non-antibody binding proteins are increasingly used as alternatives to traditional antibodies, especially in research applications where small size, stability, or animal-free production is desired. Examples include:

• DARPins (Designed Ankyrin Repeat Proteins): Engineered for high affinity and specificity to various targets.
• Monobodies: Based on the fibronectin type III domain, customizable for different targets.
• Anticalins: Derived from the lipocalin scaffold, with low immunogenicity.
• Affibodies and Affimers: Small, robust protein scaffolds used for target recognition.

These are generally discussed as alternatives to antibodies in research, not as specific partners to clone 1a3.

Conclusion

There is no evidence in the provided literature that clone 1a3 (anti-HLA-DQ) is routinely used alongside specific other antibodies or proteins. In HLA-DQ/MHC class II research, companion reagents might include antibodies against other MHC class II molecules (e.g., HLA-DR), T cell markers (e.g., CD4), or B cell markers (e.g., CD19, CD20), but these are not explicitly linked to 1a3 in the available sources. The broader field offers many alternative binding proteins, but these are generally discussed as antibody alternatives, not as specific partners for 1a3.

The key scientific finding associated with "clone 1a3" in the literature is the successful heterologous expression of active human uridine diphosphate glucuronosyltransferase 1A3 (UGT1A3) enzyme in Chinese hamster lung (CHL) cells. This was achieved by amplifying the full-length UGT1A3 gene from human liver RNA, confirming its sequence (matching the UGT1A3-3 allele), subcloning into the mammalian expression vector, and verifying both transcription and enzymatic activity in stably transfected CHL cells.

Key points from citations of clone 1a3:

• The cloned UGT1A3 gene was functionally expressed in CHL cells, as shown by RT-PCR and enzyme assays (specifically glucuronidation of quercetin).
• The expressed enzyme demonstrated active glucuronidation, confirmed by HPLC analysis and enzymatic hydrolysis.
• The research validated that recombinant UGT1A3 can be stably produced in mammalian cells, providing a platform for further biochemical and pharmacogenetic studies.
• The clone sequenced corresponded to a specific human allele (UGT1A3-3), which contains three SNPs relative to the wild-type gene.

No results indicate controversial or conflicting findings in cited literature about clone 1a3 expression or function. The findings are significant for studying drug metabolism, as UGT1A3 is an important enzyme for the glucuronidation of xenobiotics and endogenous compounds.