Anti-Influenza A Virus H1N1 Antibody (20303)

Anti-Influenza A Virus H1N1 Antibody (20303)

Product No.: 20303

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Target
Influenza A Virus H1N1
Product Type
Polyclonal
Isotype
Rabbit
Applications
WB

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Data

SDS-PAGE and Western blot analysis of 3ug intact A/California/14/2009 H1N1 virions. Influenza A virus polyclonal antibody was diluted 1:1,000.
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Antibody Details

Product Details

Reactivity Species
Influenza A Virus H1N1
Host Species
Rabbit
Immunogen
A/California/14/2009 H1N1 virions
Product Concentration
Lot Specific
Formulation
PBS, pH 7.4 with 0.02% sodium azide
State of Matter
Liquid
Product Preparation
Purified by Protein G affinity chromatography
Storage and Handling
This antibody is stable for at least one (1) year at -20°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunoblotting: use at 1:500-1:1,000 dilution

These are recommended dilutions.User should determine optimal dilutions for their application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to Influenza A Virus H1N1 HA (~75kDa), NA (~55kDa), M (~26kDa) and NP (~26-27kDa). M and NP often comigrate as a single band in SDS-PAGE. It also reacts with A/New Caledonia/20/99, and A/Brisbane/10/2007. Other isolates of H1N1 have not been evaluated.
Background
Influenza A viruses are subdivided into 16 H (H1-H16) and 9 N (N1-N9) subtypes. Nomenclature for these virus isolates indicate the influenza virus type (A or B), host species (omitted if human), geographical site, year of isolation, and the H and N subtype (example: A/California/14/2009 H1N1). The main antigenic determinants of Influenza A and B viruses are the hemagglutinin (HA) and neuraminidase (NA) transmembrane glycoproteins. NA forms a tetramer with an average size of 220kDa (~55kDa per monomer). The matrix (M) protein of Influenza A virus is one of the two group-specific internal proteins of the virus. The non-structural protein (NP) exists as a homeodimer (~52kDa) consisting of two identical monomers (each ~26kDa).

Antigen Details

Research Area
Infectious Disease

References & Citations

General Western Blot Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.