Anti-Mouse CD252 (OX40L) – Purified

Anti-Mouse CD252 (OX40L) – Purified

Product No.: C2146

[product_table name="All Top" skus="C2146"]

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Clone
RM134L
Target
OX 40 Ligand
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
OX 40 Ligand, OX40L, CD134L, TNFSF4
Isotype
IgG2b
Applications
FC
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IHC FF

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Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Rat NRK-52E cell line transfected with mouse OX-40L gene
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Product Preparation
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. Each lot of this antibody is quality control tested
Additional Applications Reported In Literature ?
Additional reported applications (for the relevant formats) include:
ICH-FFPE
B:The RM134L antibody can block the costimulatory activity of OX40L. The Purified GOLD or PLATINUM part numbers are recommended for functional assays.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone RM134L, Anti-Mouse CD252 (OX40L) recognizes an epitope on Mouse CD252.
Antigen Distribution
Activated B cells, antigen-presenting cells
Background
CD252 is a 35 kD member of the TNF superfamily and is the ligand for OX-40, which is primarily found on activated T cells. The CD252/OX-40 pair contributes to B-cell and T-cell interaction during the immune response. Both proliferative and IL-2 production responses of activated T cells are enhanced via OX-40 antigen stimulation, while proliferation and Ig secretion by activated B cells is enhanced via CD252 stimulation. Hence, because both T cells and B cells are activated in cross-linking, the CD252/OX-40 pair is considered reciprocally costimulatory.

Antigen Details

Ligand/Receptor
OX40 (CD134)
Function
B-cell/T-cell interaction, costimulation
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
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Immunology
Flow Cytometry
IHC FF

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.