Anti-Mouse CD252 (OX40L) – DyLight® 650

Anti-Mouse CD252 (OX40L) – DyLight® 650

Product No.: C2345

[product_table name="All Top" skus="C2345"]

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OX 40 Ligand
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
OX 40 Ligand, OX40L, CD134L, TNFSF4

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Product Size
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Antibody Details

Product Details

Reactive Species
Host Species
Rat NRK-52E cell line transfected with mouse OX-40L gene
Product Concentration
0.2 mg/ml
This DyLight® 650 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Product Preparation
This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Followed by purification this antibody was conjugated to DyLight 650 using optimal conditions.
Storage and Handling
This DyLight® 650 conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
Next Day 2-8°C
Excitation Laser
Red Laser (652 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µl volume. Each lot of this antibody is quality control tested.
Additional Applications Reported In Literature ?
Additional reported applications (for the relevant formats)
B:The RM134L antibody can block the costimulatory activity of OX40L.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Conjugation Details

Reporter Molecule
DyLight®650 has a maximum emission of 668 nm when it is excited at 633nm / 635nm.


Rat Anti-Mouse OX 40 Ligand (CD252) (Clone RM134L) recognizes an epitope on Mouse CD252.
Antigen Distribution
Activated B cells, antigen presenting cells
CD252 is a 35 kD member of the TNF superfamily and is the ligand for OX-40, which is primarily found on activated T cells. The CD252/OX-40 pair contributes to B-cell and T-cell interaction during the immune response. Both proliferative and IL-2 production responses of activated T cells are enhanced via OX-40 antigen stimulation, while proliferation and Ig secretion by activated B cells is enhanced via CD252 stimulation. Hence, because both T cells and B cells are activated in cross-linking, the CD252/OX-40 pair is considered reciprocally costimulatory.

Antigen Details

OX40 (CD134)
B-cell/T-cell interaction, costimulation
NCBI Gene Bank ID
Research Area
Costimulatory Molecules
Flow Cytometry

Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.