Anti-Mouse CD80 [Clone 16-10A1] — Purified in vivo PLATINUM™ Functional Grade

The 16-10A1 clone is a monoclonal antibody widely used in in vivo mouse studies to block the costimulatory activity of CD80 (B7-1), thereby modulating immune responses, particularly T-cell activation.

• Mechanism of Action: 16-10A1 binds specifically to mouse CD80, a costimulatory molecule expressed on activated B cells, dendritic cells, and macrophages. By blocking CD80, it prevents its interaction with CD28 (which stimulates T cell activation and survival) and CTLA-4 (which inhibits T cell responses), but the main use is to disrupt the positive costimulatory signal necessary for full T-cell activation.

• Functional Uses:

  • Blocking CD80 Costimulation: In in vivo mouse studies, 16-10A1 is typically injected to experimentally block CD80-mediated costimulation of T cells. This is used to study the role of CD80 in immune activation, tolerance, autoimmune models, graft vs. host disease, transplant tolerance, and tumor immunity.
  • Selective Activity: Importantly, 16-10A1 blocks CD80 without affecting CD86 (B7-2), allowing researchers to dissect the individual contributions of these two costimulatory molecules.

• Administration: The antibody is administered systemically in mice, usually via intraperitoneal or intravenous injection, at doses determined by experimental design and prior literature. Low-endotoxin, in vivo–grade preparations are chosen to avoid immune activation from contaminants.

• Experimental Outcomes: Using 16-10A1, researchers can suppress antigen-specific immune responses, reduce T cell proliferation, or alter disease models that depend on CD80-mediated T cell costimulation. For example, blocking CD80 can attenuate certain autoimmune or inflammatory responses in mouse models.

In summary, clone 16-10A1 is a tool antibody for blocking CD80 in live mice to investigate the consequences of inhibiting this key costimulatory pathway on immunity and disease.

The correct storage temperature for sterile packaged clone 16-10A1 (anti-CD80 antibody) is between 2°C and 8°C (refrigerated), undiluted, and protected from prolonged exposure to light. It should not be frozen.

This recommendation is consistent across multiple suppliers and formulations (including PE-conjugated, APC-conjugated, and biotinylated forms), indicating it is standard for this monoclonal antibody clone. Always check the accompanying product-specific datasheet for formulation-specific advice.

Commonly used antibodies or proteins studied alongside 16-10A1 (anti-CD80, B7-1) in the literature typically include antibodies against CD86 (B7-2), CD28, CTLA-4 (CD152), and markers for B cells, T cells, or antigen-presenting cells.

Essential context and supporting details:

• CD86 (B7-2): CD86 is closely related to CD80 and also interacts with CD28 and CTLA-4, making antibodies against CD86 a frequent companion in studies using 16-10A1 to investigate costimulatory pathways.
• CD28: As the principal receptor for the co-stimulatory signal provided by CD80 and CD86, antibodies against CD28 are often used to dissect T cell activation mechanisms.
• CTLA-4 (CD152): Since CTLA-4 competes with CD28 for binding to CD80 and CD86, anti-CTLA-4 antibodies are important for studying inhibitory regulation of T cell responses alongside 16-10A1.
• Other cell surface markers: Antibodies specific for T cell, B cell, or APC markers (e.g., CD3, CD19, MHC II) are frequently included in experimental panels to identify and characterize immune cell subsets in flow cytometry or immunohistochemistry applications.

Additional relevant information:

• Functional assays: Studies using 16-10A1 may involve blocking co-stimulation (using anti-CD80 and anti-CD86), immunoprecipitation (with anti-CD80 and related pathway inhibitors), and staining for cell activation or cytokine expression (with additional antibodies or proteins as functional readouts).
• Published protocols: Literature frequently combines 16-10A1 with anti-CD86 (clone GL-1), anti-CTLA-4, anti-CD28, anti-PD-1, and anti-CD3, depending on the immunological question and experimental design.
• Rationale for combination: These combinations are used to tease apart T cell costimulation, regulation of immune response, and the effects of manipulating CD80-mediated pathways.

In summary, the antibodies most commonly used with 16-10A1 in immunology research target CD86, CD28, CTLA-4, and major immune cell markers, reflecting the central role of CD80 in T cell costimulation and immune regulation.

Clone 16-10A1 is a monoclonal antibody extensively cited in scientific literature for its specificity in targeting mouse CD80 (B7-1) and its ability to block CD80-mediated costimulatory signals during T cell activation, with no effect on CD86. Key findings from its citations include:

• Blocking CD80 Costimulation: The 16-10A1 clone is widely used to functionally block CD80 in vitro and in vivo, allowing researchers to dissect the role of CD80–but not CD86–in T cell activation, tolerance, and immunoregulatory networks. It specifically disrupts CD80’s interaction with both CD28 and CTLA-4, yielding insights into immune checkpoint regulation.

• Cellular Expression Patterns: CD80 detected using 16-10A1 is present on activated B cells, T cells, monocytes, macrophages, and dendritic cells in mice. This pattern underpins its use in characterizing immune responses and cell populations.

• Diagnostic Utility in Dogs: While primarily designed for mice, recent studies report that clone 16-10A1 can also bind to neutrophils and monocytes in healthy dogs and on tumor cells in canine acute myeloid leukemia (AML), making CD80 a potential flow cytometric marker for AML in veterinary diagnostics. However, this has revealed discrepancies: the 16-10A1 clone shows broader reactivity in dogs compared to previous reports, raising questions about possible cross-reactivity with other B7 family molecules (such as CD86 or CD74).

• Application-Specific Performance: 16-10A1 is commonly used across a variety of applications, including:

  • Functional in vivo blockade of CD80
  • Flow cytometric phenotyping of activated immune cell subsets
  • Immunoprecipitation and immunohistochemistry for mechanistic and localization studies

• Molecular Target Specificity: Structural and binding studies confirm that 16-10A1 targets the epitope on CD80 without disrupting other ligand or antibody interactions, validating its use in competitive assays and functional experiments.

• Technical Notes: The antibody is produced as an Armenian hamster IgG, typically purified via affinity methods and is suitable for both research and pre-clinical functional studies in mice.

In summary, clone 16-10A1 is highly valued for its specificity and blocking function against CD80 in murine models, but findings from cross-species applications (e.g., dogs) highlight differences in reactivity and emphasize the importance of clone selection and validation across species and experimental protocols.