Anti-MyoD (Phospho-Ser200) Antibody (43077)
Data
Immunoblotting: Detection of MyoD in extracts of HeLa cells untreated or treated with EGF. - -
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Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of Serine 200 (A-S-S(p)-P-R) derived from human MyoD and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~40kDa is detected.
These are recommended working dilutions. End users should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to MyoD (Phospho-Ser200) Background MyoD encodes a nuclear protein that is a member of the basic helix-loop-helix family of transcription factors and the myogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest which is required for myogenic initiation. MyoD is also involved in muscle regeneration. It activates its own transcription which may stabilize the commitment to myogenesis. Antigen DetailsResearch Area Phosphospecific Antibodies References & CitationsTechnical Protocols |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
