Goat F(ab’)₂ Anti-Rabbit IgG (H&L) (Adsorbed Against: Hu., Bv.) – Texas Red

Purified Rabbit IgG (H&L) (>98%)

This secondary antibody is formulated inin 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA, 1.0% glycerol and 0.09% sodium azide as a preservative. The specific antibody concentration of this conjugate is 1.0 mg per ml.

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

Stable when stored at 2 - 8°C. Do not freeze.

Next Day 2-8°C

The suggested working dilution for immunohistology is approximately 1:100. However, each investigator should determine their own optimal working dilution for each specific research application.

Goat F(ab')₂ Anti-Rabbit IgG (H&L) recognizes Rabbit IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

1. Fujiki, Y. et al. (2002) J Biol Chem. 277(46):44513-24. Article Link
2. Fujiki, Y. et al. (2000) J Biol Chem. 275(28):21703-14. Article Link
3. Fujiki, Y. et al. (2000) J Biol Chem. 275(28):21715-21. Article Link