Anti-Mouse CD11c [Clone N418] — Purified in vivo GOLD™ Functional Grade

Clone N418, an anti-mouse CD11c antibody, is widely used in in vivo mouse studies for several key applications:

1. Targeting Dendritic Cells: N418 is highly specific for dendritic cells in mice, which express CD11c, a component of the integrin receptor complex. This specificity makes it an effective tool for depleting dendritic cells in vivo, providing valuable insights into the immune system and disease mechanisms.

2. Enhancing Antigen-Specific Responses: N418 has been reported to enhance antigen-specific responses when used to target dendritic cells in vivo. This property can be beneficial in studying immune responses and vaccine efficacy.

3. Applications in Functional Assays: N418 is used in various assays such as ELISA, flow cytometry, neutralization, and functional assays like bioanalytical PK and ADA assays. These applications help in studying biological pathways affected by the mouse CD11c protein.

4. Depletion Studies: By depleting dendritic cells, researchers can study their role in immune regulation and disease progression. This approach has been crucial in understanding immune function and developing therapeutic strategies.

Overall, clone N418 is a versatile tool in mouse immunology research, offering a robust method for studying dendritic cell biology in vivo.

The correct storage temperature for sterile packaged clone N418 (anti-mouse CD11c antibody) depends on the storage duration:

• For long-term storage (up to 12 months): Store at -20°C to -70°C.
• For short-term storage (up to 1 month): Store at 2°C to 8°C.

Additional details:

• If aliquoting after thawing, keep working aliquots at 2–8°C for short-term use (up to 4 weeks) and store remaining aliquots at -20°C.
• Protect from prolonged exposure to light and avoid repeated freeze-thaw cycles.
• Do not store in frost-free freezers, as temperature cycling can degrade antibody quality.

This guidance assumes standard formulations (typically PBS-buffered solutions) and sterile packaging for research use. Always refer to the specific product datasheet or manufacturer’s instructions for your particular lot.

Commonly used antibodies or proteins with N418 (an anti-CD11c antibody for dendritic cell labeling) in the literature include markers for dendritic cell subsets, T cell markers, other myeloid lineage markers, and proteins relevant to immune function.

Key examples from the literature:

• CD8 and CD4: Often co-stained in immunological studies to distinguish T cell subsets when analyzing dendritic cell interactions.
• MHC class II (IA/IE): Used to confirm antigen-presenting cell phenotype alongside CD11c.
• CD11b: Marks myeloid cells; combined with N418 to distinguish conventional dendritic cells (cDCs) from other myeloid populations.
• F4/80: A macrophage marker, sometimes used to exclude contaminating cells in dendritic cell preps.
• Other lineage markers: Such as B220 (B cells), Ly6G (neutrophils), Ly6C (monocytes), and NK1.1 (natural killer cells), for gating strategies in flow cytometry.
• Reporter antigens and secondary antibodies: In targeting or tracking experiments, goat anti-hamster antibody, biotinylated antigens, and anti-biotin antibodies are used respectively with N418.

Typical combinations in experiments:

• In targeted immunization protocols, N418 is often used with antigen-specific antibodies, controls such as rat J1.2, and secondary antibodies (e.g., goat anti-hamster IgG) to distinguish specific targeting effects.
• For multiplexed imaging, antibodies to structural or activation markers, such as Ki-67 or phosphorylated signaling proteins, may be included, but these are chosen based on experimental goals.

Summary Table: Frequently Combined Antibodies/Proteins with N418

These combinations help researchers refine the specificity of dendritic cell identification, distinguish functional subsets, and ensure accurate exclusion/inclusion of cell populations in immunological experiments. If you need combinations specific to a particular experimental technique (e.g., flow cytometry vs. immunofluorescence), please specify for more focused examples.

Clone N418 is a monoclonal antibody clone that recognizes CD11c, a cell surface marker primarily found on dendritic cells and other myeloid cells. This clone has been extensively used in immunological research, with several key findings emerging from studies that have cited its use.

Primary Applications and Characterization

Clone N418 has been widely utilized for flow cytometry, immunohistochemistry, and functional studies involving CD11c-positive cells. The clone is particularly valuable for identifying and characterizing dendritic cells in various experimental models, as CD11c serves as a reliable marker for this critical immune cell population.

Stress-Induced Immunosuppression Research

One significant finding from studies using clone N418 involves the investigation of psychological stress effects on immune function. Research has demonstrated that social disruption stress creates an immune suppressive environment, with clone N418 being used to identify and analyze CD11c-positive dendritic cells in these stress models. This work has contributed to understanding how psychological stressors can compromise immune surveillance and response mechanisms.

Cancer Immunotherapy Studies

Clone N418 has been instrumental in cancer immunotherapy research, particularly in studies examining anti-PD-1 treatment efficacy. Recent findings have revealed that effective anti-PD-1 therapy enhances tumor-infiltrating lymphocytes, with dendritic cells identified using clone N418 playing crucial roles in orchestrating anti-tumor immune responses.

In these studies, researchers discovered that perforin expression across multiple lymphocyte subsets showed strong negative correlations with tumor volume, suggesting that perforin may serve as a reliable marker of anti-PD-1 efficacy. Unexpectedly, the research revealed that anti-PD-1 treatment increased cytotoxic markers in traditionally non-cytotoxic cell populations, including regulatory T cells and B cells.

Functional Immune Cell Analysis

Studies utilizing clone N418 have contributed to understanding the functional status of tumor-infiltrating lymphocytes. The research has shown significant increases in cytotoxic markers (CD107a, granzyme B, and perforin) across various immune cell populations following anti-PD-1 treatment, with clone N418 helping to identify the dendritic cell populations that coordinate these responses.

Technical Applications

Clone N418 has proven valuable for in vivo functional studies, with purified versions available for direct injection and functional analysis. The clone has been successfully used across multiple experimental approaches, including flow cytometry, immunohistochemistry on frozen sections, immunofluorescence, and immunoprecipitation studies.

The collective findings from clone N418 citations demonstrate its importance in advancing our understanding of dendritic cell biology, stress immunology, and cancer immunotherapy mechanisms, making it a cornerstone tool in modern immunological research.