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Anti-Human CD3 (CD3) Purified Low Endotoxin Functional Formulation

Monoclonal Antibody


Prod. No.:C364
Clone:UCHT-1
Isotype:Mouse IgG1
Conc.:1.0 mg/ml
Pkg. Size:0.5 mg, 1.0 mg, 5.0 mg, 25 mg, 100 mg
Starting at:$98.00
Availability:In Stock
Storage:Sterile 2-8°C Detailed storage instructions below.
Description
Specificity:
Mouse Anti-Human CD3 (Clone UCHT-1) recognizes Human CD3. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Anti-Human CD3 recognizes a (Mr 22-28 kDa) T-cell surface glycoprotein.1 The epitope recognized by the CD3 antibody is expressed on a constant region of the epsilon chain of the CD3 antigen/T-cell receptor complex (TCR).2

Antigen Distribution:
The CD3 antigen is expressed on approximately 60-80% of human peripheral blood lymphocytes, 20-40% of splenic lymphocytes, the majority of T-CLL and approximately 70% of T-ALL Cells.

Background:
Anti-CD3 may be used for enumerating immunocompetent T-lymphocytes in peripheral blood. Clone UCHT-1 is also useful in histology for localization of T-lymphocytes in tissue and may be used to enrich T-cells by cell sorting. This T-cell marker has also been used to determine T-versus B-cell lymphomas and leukemias.

Known Species Cross Reactivity: Hu+
Host Species
Mouse
Formulation
This antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2, 150 mM NaCl with no carrier protein, potassium or preservatives added.
Purity
>98% by SDS-PAGE and HPLC
Endotoxin
<0.1 EU/µg as determined by the LAL method
Storage and Stability
This antibody is stable for at least one week when stored sterile at 2-8°C. For long term storage aseptically aliquot in working volumes without diluting and store at –80°C. Avoid Repeated Freeze Thaw Cycles.
Reported Applications
FC, IP, WB
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD3. A suggested method would be to stain cells expressing CD3 with 2.0 µg per million cells in a total staining volume of 100 µl followed by Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189).
Each investigator should determine their own optimal working dilution for specific applications.


Country of Origin
USA
References
1. Meuer, S. C. et al. (1983) Nature 303:808
2. van Dongen, J. J. M. et al. (1988) Blood 71:603
3. Beverly, P. C. L. et al. (1981) Eur. J. Immunol. 11:329
4. Leukocyte Typing: 3rd Workshop: Code No. 126, 208 and 471; 4th Workshop: Paper No. T3.2
Related Products
Goat Anti-Mouse IgG (H&L)-R-phycoerythrin (Leinco Prod. No.: M1189)
Anti-Human CD3-Biotin (Leinco Prod. No.: C106)
Anti-Human CD3-R-phycoerythrin (R-PE) (Leinco Prod. No.: C108)
Anti-Human CD3-Allophycocyanin (APC) (Leinco Prod. No.: C309)
PN:
Anti-Human CD3 Purified (Leinco Prod. No.: C105)
Anti-Human CD3-Fluorescein (FITC) (Leinco Prod. No.: C107)

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