Anti-Mouse CD120α (TNFR1) [Clone 55R-170] — Purified in vivo GOLD™ Functional Grade

Clone 55R-170 is primarily used in in vivo mouse studies to block or neutralize the bioactivity of mouse TNF Receptor I (TNFRI), also known as CD120a. This function enables researchers to study the role of TNFRI-mediated signaling in models of inflammation, infection, and TNF-related pathology.

Supporting details:

• Mechanism: 55R-170 is a monoclonal antibody targeting the extracellular domain of mouse TNFRI. In vivo, it acts as a TNF antagonist, specifically inhibiting TNF binding to the receptor, thereby disrupting downstream signaling.
• Experimental usages:
  • Protection from inflammation: Administration of 55R-170 protects mice from lethal endotoxin (septic shock) by blocking TNFRI signaling.
  • Infectious disease: It has been used to block development of protective response against Listeria monocytogenes infection, suggesting TNFRI's role in immune memory and resistance.
  • Skin necrosis: Both anti-p55 (55R-170) and anti-p75 antibodies can block TNF-induced skin necrosis in mice.
• Other applications:
  • Flow cytometry and ELISA: 55R-170 is also used for flow cytometric analysis and as a detection antibody in ELISA assays measuring TNFRI levels or occupancy.
  • Functional grade: Purified formats suitable for in vivo studies are available to ensure low endotoxin and high specificity for murine TNFRI.

Key insights:

• 55R-170 is specific to mouse TNFRI and does not cross-react with p75 TNF receptor, allowing selective intervention in TNFRI signaling pathways in vivo.
• Researchers employ 55R-170 when they need to study how TNF-mediated responses depend specifically on TNFRI rather than TNFRII, such as dissecting roles in inflammation, immune response, and cell death.

If you need details on dosing, administration routes, or particular disease models, consult published protocols or the referenced studies for specifics.

The correct storage temperature for sterile packaged clone 55R-170 (Anti-TNFRSF1A antibody) depends on your intended storage duration:

• For short-term storage (up to 8 weeks), store at 2–8°C (standard refrigerator).
• For long-term storage, aliquot aseptically into working volumes and store at -20°C to -70°C (manual defrost freezer).
• For maximum preservation (over a year or prolonged periods), storage at ?-70°C is advised.

Avoid repeated freeze-thaw cycles, as these can reduce antibody activity.

Summary of best practices:

• 2–8°C: short-term
• -20°C to -70°C: long-term
• Aliquot to avoid freeze-thaw
• Store at the back of the freezer/refrigerator for the most stable temperature

Always consult the lot-specific datasheet for manufacturer recommendations, as procedures may periodically change.

Commonly used antibodies or proteins with 55R-170 (anti-mouse TNFRI):

55R-170 is an antibody specific for mouse TNF Receptor I (TNFRI; p55; CD120a) and is widely used to study TNF signaling and immune responses. In the literature, it is frequently paired or compared with several other antibodies and proteins:

• Anti-mouse TNFRII (p75) antibodies: These are often used in parallel to dissect the specific contributions of TNFRI (targeted by 55R-170) and the p75 receptor in TNF signaling.

  • Example: Monoclonal antibodies specific for murine p75 TNF receptor are used to study differential effects or to create sandwich ELISAs with anti-p55 reagents like 55R-170.

• 55R-286: Another monoclonal antibody against mouse TNFRI. In ELISA, 55R-286 is commonly used as the capture antibody while 55R-170 (often biotinylated) is used as the detection antibody to quantify soluble TNFRI, especially in mouse serum after LPS or cytokine challenge.

• Ligands TNF-? and LT-? (also called TNF-?): These are natural ligands for TNFRI; recombinant forms are sometimes used in co-treatments to probe receptor function when using blocking antibodies like 55R-170.

• Nitric oxide synthase inducers, LPS (lipopolysaccharide), and cytokine panels: In functional assays, these are used alongside 55R-170 to assess how TNFRI neutralization affects downstream signaling, cytokine profiles, or inflammation in mouse models.

• Isotype control antibodies: To control for non-specific binding in flow cytometry or ELISA studies with 55R-170.

Summary table:

Key references supporting these pairings include BioLegend and DTIC technical reports detailing ELISA design and functional immunology studies, as well as peer-reviewed articles identifying the use of p75- and p55-specific antibodies in combination for pathway elucidation.

Key findings from scientific literature citing clone 55R-170 focus on its role as a monoclonal antibody that specifically binds and antagonizes mouse TNF Receptor I (p55, TNFRI, CD120a), with important applications in both basic and disease-related immunological research.

Essential findings and supporting details:

• 55R-170 is a TNF antagonist: This antibody binds to the mouse TNFRI and blocks its signaling, both in vitro and in vivo, making it a powerful tool to dissect the specific functions of TNF signaling through the p55 receptor.

• Functional antagonism supports research on TNF biology: Research using 55R-170 has demonstrated that blocking TNFRI:

  • Inhibits TNF-induced cytotoxicity in target cells (such as L929 fibroblasts) with high efficacy.
  • Clarifies the distinct roles of p55 and p75 TNF receptors by selectively inhibiting p55-dependent processes without off-target effects on p75.

• In vivo effects and experimental models:

  • 55R-170 has been used to protect mice from lethal endotoxin shock, highlighting the receptor's key involvement in inflammatory responses.
  • It blocks both the pro-inflammatory and immunosuppressive activities mediated via TNFRI, and has been applied in models of infection, autoimmune disease, and cancer immunotherapy.
  • The antibody has shown utility in blocking the development of skin necrosis in mice exposed to high doses of TNF.

• Key experimental observations:

  • 55R-170 and related antibodies enabled precise mapping of TNFRI's necessity for the upregulation of cell-adhesion molecules (such as VCAM-1, ICAM-1, and E-selectin) in response to TNF in mouse pulmonary endothelial cells.
  • 55R-170 does not cross-react with p75 (TNFRII), ensuring the specificity of research results.

• Role in immunotherapy research:

  • Studies reveal that TNFRI blockade (as achieved by 55R-170) can synergize with anti-PD-1 immunotherapies in cancer models, overcoming resistance by modulating TNF/TNFRI-related immune responses.

Summary of core scientific impact:

• 55R-170 is a validated, function-blocking, and highly specific monoclonal antibody for mouse TNFRI, enabling precise mechanistic studies of TNF signaling and therapeutic manipulation of the TNF pathway in mouse models. Its use has elucidated the unique roles of TNFRI in inflammation, cell death, immune regulation, and response to biological therapies.