Anti-Mouse CD120a (TNFR1) (Clone 55R-170) – Purified In vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.T950
Clone
55R-170
Protein
TNFR1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
TNFR-I, TNFRSF1A, P55, P60
Isotype
Armenian Hamster
IgG
Applications
B
,
FC
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
T950-1.0 mg
1.0 mg
$198.00
In stock
Max:
Min: 1
Step: 1
T950-5.0 mg
5.0 mg
$355.00
In stock
Max:
Min: 1
Step: 1
T950-25 mg
25 mg
$1,025.00
In stock
Max:
Min: 1
Step: 1
T950-50 mg
50 mg
$1,650.00
In stock
Max:
Min: 1
Step: 1
T950-100 mg
100 mg
$2,220.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Immunogen
Purified Recombinant Mouse TNFR1 (>98%)
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.
WB To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:A238 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.
FC
Other Applications Reported In Literature ?
IP 2 µg/mg of protein lysate
B
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 55R-170 recognizes mouse CD120a.
Antigen Distribution
CD120a is constitutively expressed in most tissues.
Background
Tumor Necrosis Factor Receptor Type I (TNFRI) is a 55kD Type I transmembrane protein. TNFRI is expressed independently of TNFRII at low levels on a wide variety of cell types. TNFRI binds TNF-α and TNF-ß (also known as LT-α). The subsequent signaling is known to be important for inducing cytolytic activity, antiviral activity, expression of manganous superoxide dismutase and intercellular adhesion molecule (ICAM), IL-6 mRNA accumulation and NF-ĸB induction. TNFRI carries an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein). Binding of 55R-170 antibody to TNFRI has been shown to block in vitro and in vivo receptor signaling initiated by ligand binding.

Antigen Details

Protein
Ligand/Receptor
TNF-α, LT-α (TNF-β)
Function
Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
PubMed
NCBI Gene Bank ID
Research Area
Immunology
.
Innate Immunity

References & Citations

1. Dana, R. et al. (2000) Arch Ophthalmol. 118: 1666
2. Donner, DB. et al. (2008) J Immunol. 181: 1288
3. Pasparakis, M. et al. (2008) Nat Immunol. 9: 1015
Flow Cytometry
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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