Anti-Mouse CD120a (TNFR1) (Clone 55R-170) – Purified in vivo GOLD™ Functional Grade

Pricing & Details

Product No.T250
Clone
55R-170
Protein
TNFR1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD120α, TNFRSF1A, P55, P60
Isotype
Armenian Hamster
IgG
Applications
B
,
IP
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
T250-1.0 mg
1.0 mg
$105.00
In stock
Max:
Min: 1
Step: 1
T250-5.0 mg
5.0 mg
$355.00
In stock
Max:
Min: 1
Step: 1
T250-25 mg
25 mg
$965.00
In stock
Max:
Min: 1
Step: 1
T250-50 mg
50 mg
$1,595.00
In stock
Max:
Min: 1
Step: 1
T250-100 mg
100 mg
$2,300.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Armenian Hamster
Immunogen
Purified Recombinant Mouse TNFR1 (>98%)
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 55R-170 recognizes mouse CD120a.
Antigen Distribution
CD120a is constitutively expressed in most tissues.
Background
Tumor Necrosis Factor Receptor Type I (TNFRI) is a 55kD Type I transmembrane protein. TNFRI is expressed independently of TNFRII at low levels on a wide variety of cell types. TNFRI binds TNF-α and TNF-ß (also known as LT-α). The subsequent signaling is known to be important for inducing cytolytic activity, antiviral activity, expression of manganous superoxide dismutase and intercellular adhesion molecule (ICAM), IL-6 mRNA accumulation and NF-ĸB induction. TNFRI carries an approximately 80 amino acid death domain near its carboxy terminus capable of transmitting an apoptotic signal through its interaction with TRADD (TNF Receptor I associated death domain protein). Binding of 55R-170 antibody to TNFRI has been shown to block in vitro and in vivo receptor signaling initiated by ligand binding.

Antigen Details

Protein
Ligand/Receptor
TNF-α, LT-α (TNF-β)
Function
Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
PubMed
NCBI Gene Bank ID
Research Area
Immunology
.
Innate Immunity

References & Citations

1. Dana, R. et al. (2000) Arch Ophthalmol. 118: 1666
2. Donner, DB. et al. (2008) J Immunol. 181: 1288
3. Pasparakis, M. et al. (2008) Nat Immunol. 9: 1015
Immunoprecipitation Protocol
General Western Blot Protocol

Formats Available