Anti-Mouse CD120a (TNFR1) [Clone 55R-170] — Purified in vivo PLATINUM™ Functional Grade

Clone 55R-170 is a monoclonal antibody targeting mouse TNF Receptor I (CD120a, TNFRI) and is used in in vivo mouse studies to modulate, block, or detect TNFRI-mediated signaling.

• Primary applications in vivo:

  • Functional blockade/neutralization: 55R-170 is directly injected into mice to neutralize TNFRI bioactivity and thereby inhibit TNF-alpha signaling through this receptor. This approach is used to study the role of TNFRI in inflammation, immune response, and disease models (e.g., endotoxin shock, infection, autoimmunity).
  • Flow cytometry and detection: The antibody may be used to stain cells from mouse tissues to directly quantify TNFRI expression ex vivo, but blocking activity is the major in vivo usage.
  • ELISA (detection): In assay setups, 55R-170 is used as a detecting antibody for quantifying soluble TNFRI in mouse samples; however, this is usually done ex vivo.

• Key features for in vivo use:

  • Purified to low endotoxin levels to minimize nonspecific immune activation.
  • Reacts specifically with mouse TNFRI, not other TNF receptors.
  • Often used alongside other tools (e.g., knockout mice, ligands) to dissect TNFRI-dependent pathways.

• Representative in vivo experimental uses:

  • Blocking TNFRI protects mice from lethal endotoxin shock: Administration of TNFRI-blocking antibodies (such as 55R-170) can protect against TNF-mediated pathology and has been cited in studies analyzing immune and inflammatory responses.
  • Immunological disease models: Used to probe TNFRI roles in autoimmune disease, infection (by blocking protective or pathogenic TNF signaling), and other pathological states.

In summary, clone 55R-170 is widely used in vivo to neutralize TNFRI function in the mouse, enabling direct study of TNF-alpha/TNFRI biology and related immune mechanisms.

The correct storage temperature for the sterile packaged monoclonal antibody clone 55R-170 is 2–8°C for short-term storage (up to 8 weeks or 1 month), and -20°C to -70°C (or below) for long-term storage.

• Short-term storage (up to 1 month): Store at 2–8°C (refrigerator temperature).
• Long-term storage: Aseptically aliquot into working volumes and store at -20°C to -70°C (or lower).
• Avoid repeated freeze-thaw cycles to maintain antibody stability.

Always check the specific lot datasheet for any updated or product-specific instructions, as recommendations may vary slightly by manufacturer.

Commonly used antibodies and proteins in the literature alongside 55R-170 (an anti-mouse TNF receptor type I/TNFRI/p55 antibody) include other TNF receptor-specific antibodies, particularly those against the p75 (TNFRII) receptor, as well as antibodies aimed at detecting TNF-? and LT-? (TNF-?), which are ligands for TNFRI.

Key details from the literature:

• p75 (TNFRII) antibodies: Studies utilizing 55R-170 often use antibodies that specifically recognize the p75 TNF receptor (sometimes called TNFRII) to differentiate and analyze the distinct roles of the two main TNF receptors. For example, epitope mapping and ELISA development often pair p55- and p75-specific monoclonals.

• Other p55 TNFR-specific monoclonals: Several clones besides 55R-170, notably 55R-176, are used for functional comparison, as both are TNF antagonists. 55R-593 is another p55-specific antibody with agonist activity, utilized in mechanistic studies to contrast agonistic and antagonistic functions.

• Anti-TNF-? and anti-LT-? antibodies/proteins: Since TNFRI binds both of these cytokines, their detection (by antibodies targeting TNF-? or LT-?) or blockade is important in relevant signaling and functional assays.

• Detection antibody pairs for ELISA: When 55R-170 is biotinylated, it is frequently used as a detection antibody in ELISA, often paired with another monoclonal such as 55R-286 for capture.

• Signal transduction proteins: Proteins involved in TNFRI signaling, such as TRADD (TNF Receptor I Associated Death Domain protein) and RIP (Receptor-Interacting Protein), may also be detected or manipulated in experiments to investigate downstream effects.

Summary Table: Common Antibodies/Proteins Used in Combination with 55R-170

These combinations facilitate functional studies—such as blocking, receptor mapping, cytokine measurement, and pathway analysis—needed to dissect TNF receptor-mediated signaling and biological effects in health and disease models.

Clone 55R-170 is a monoclonal antibody that specifically targets mouse TNF Receptor I (p55, TNFRSF1A, CD120a) and is widely used as a functional neutralizing antibody in scientific research. Its key findings and contributions in the literature are as follows:

• Neutralization and Inhibition of TNFRI Signaling:
  55R-170 effectively blocks the biological activity of mouse TNF-alpha by inhibiting TNFR I signaling, both in vitro and in vivo. It achieves ~50% inhibition of TNF-alpha-induced cytotoxicity in L929 cells at 25 ?g/mL. This ability makes 55R-170 valuable for dissecting TNF-alpha functions and downstream cellular responses.

• Dissection of TNF Receptor Functions:
  Use of 55R-170 enables researchers to distinguish roles of TNFRI from TNFRII (p75) in inflammation and immune responses. For example, studies have shown that TNFRI (p55) signaling is crucial for the upregulation of adhesion molecules (E-selectin, VCAM-1, ICAM-1) in response to TNF stimulation, which is directly confirmed by both genetic knockout models and blockade experiments using 55R-170. In particular, TNFRI blockade results in a pronounced reduction in cytokine-induced expression of these molecules, impacting leukocyte recruitment and inflammatory processes.

• Protection Against Endotoxin Shock and Infection:
  In vivo, p55-specific antibodies like 55R-170 protect mice from lethal endotoxin shock and block development of protective immune responses against Listeria monocytogenes infection, highlighting the central role of TNFRI in mediating systemic TNF-alpha effects. In contrast, TNFRII blockade alone is less effective against these systemic outcomes.

• Experimental Applications:
  55R-170 is validated for multiple laboratory techniques, including ELISA, flow cytometry, and functional neutralization assays. It is functionally tested to ensure specificity and low endotoxin content, important for reliable results in cell culture and animal models.

• No Cross-reactivity with TNFRII:
  The antibody is specific for TNFRI and does not cross-react with TNFRII, allowing for selective interrogation of receptor subtype pathways.

In summary:
The principal scientific utility of clone 55R-170 lies in its function as a potent and specific antagonist of mouse TNFRI, yielding insights into TNF-alpha biology—including receptor-dependent regulation of inflammation, cell adhesion, and systemic shock responses—through both direct receptor blockade and combination with genetic knockout models.