Anti-West Nile Virus (Clone: WNV-99)

Pricing & Details

Product No.LT567
Clone
WNV-99
Protein
West Nile virus
Formats AvailableView All
Product Type
Recombinant Monoclonal Antibody
Alternate Names
WNV
Isotype
Human IgG1
Applications
ELISA
,
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
LT567-250 µg
250 µg
$390.00
Pre-order
Max:
Min: 1
Step: 1
LT567-1.0 mg
1.0 mg
$1,400.00
Pre-order
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
West Nile
Virus
Expression Host
HEK-293
Immunogen
B cells were isolated and sequenced from an individual previously infected with a laboratory-confirmed, symptomatic WNV infection whose serum reacted to recombinant WNV NS12.
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
Formulation
This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one year. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≥ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Standard Overnight on Blue Ice.
Other Applications Reported In Literature ?
ELISA
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
WNV-99 activity is directed against the wing, flexible loop of NS1. During the screening phase and post characterization clone WNV-99 was found to bind to solid-phase recombinant WNV NS1 protein and to NS1 expressed on the surface of intact WNV-infected Vero cells. Plus, also engaged the hexameric form of NS1. WNV-99 was cross-reactive against Japanese Encephalitis Virus and Tick-borne Encephalitis Virus in a direct ELISA using recombinant NS1 proteins.

Background
West Nile Virus (WNV) is a mosquito-borne, enveloped, positive-stranded RNA flavivirus1. Flavivirus nonstructural protein NS1 has been proposed as an antibody target to avoid antibody-dependent enhancement2. NS1 is a 46-55 kDa glycoprotein that is expressed as a dimer on the cell surface and as a soluble hexamer in the extracellular space and in circulation during infection. WNV NS1 dimer consists of a ß-roll, wing, and ß-ladder.

Antigen Details

Research Area
Infectious Disease
.
Viral
.
West Nile

References & Citations

1. Goo L, Debbink K, Kose N, et al. Nat Microbiol. 4(1):71-77. 2019.
2. Wessel AW, Doyle MP, Engdahl TB, et al. mBio. 12(5):e0244021. 2021.
Elisa Sandwich Protocol
Flow Cytometry

Formats Available

Prod No.
Description
LT567
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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