Data
Detection of c-myc-tagged Protein by western blot. Samples: 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibodies: Affinity purified, goat anti-Myc Tag antibody 18824 used for WB at 0.04 µg/ml (1:25,000). Detection: Chemiluminescence with an exposure time of 30 seconds. - -
- -
Antibody DetailsProduct DetailsHost Species Goat Immunogen Amino acids 410-419 (EQKLISEEDL) of human myc conjugated to KLH. Product Concentration 1.0 mg/ml Formulation Phosphate Buffered Saline (PBS) containing 0.09% Sodium Azide State of Matter Liquid Product Preparation Purified by peptide immuno-affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at 2-8°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at a dilution of 1:1,000-1:20,000. Detection of c-myc-tagged protein in 200, 100, and 50ng of E. coli lysate containing tagged fusion protein with #18824 diluted 1:25,000.
Immunocytochemistry: use at a dilution of 1:100-1:400. ELISA: for detection use at a dilution of 1:1,000-1:30,000; for coating use at a dilution of 1:100- 1:500. Immunoprecipitation: use at 1-4ug/mg lysate These are recommended dilutions. Endusers should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Goat Polyclonal Antibody specific to c-myc. Antibody was affinity-purified on the peptide. Background c-myc is an epitope tag often constructed on the N- or C-terminus of a protein to allow for detection and analysis of the protein. The peptide sequence of the myc tag is EQKLISEEDL (Glu-Gln-Lys-Leu-Ile-Ser-Glu-Glu-Asp-Leu). The c-myc tag is approximately 1202 Daltons in atomic mass and includes 10 amino acids. Antigen DetailsFunction Transcription factor that binds DNA in a non-specific manner, yet also specifically rUniProtKB:P01108, PubMed:20010808, PubMed:24940000, PubMed:25956029}. NCBI Gene Bank ID UniProt.org Research Area Epitope Tag References & CitationsTechnical Protocols ICC   |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
