A-20 Whole Cell Lysate

Pricing & Details

Product No.A232
Formats AvailableView All
Product Type
Cell Line Lysate
Prod No.
Size
Price
Avail.
Qty
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A232-0.1 mg
0.1 mg
$124.00
In stock
Max:
Min: 1
Step: 1

Details

Cell Line
A-20 (B Lymphocyte; Reticulum Cell Sarcoma)
Tissue Type
Tumor
Growth Media
RPMI 1640 Medium with 2 mM L-Glutamine & 10% FBS (Fetal Bovine Serum)
Preparation
A-20 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Host
Mouse
Concentration
2.0 mg/ml
Formulation
The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.
Expiration Date
One year from date of receipt
Storage
This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. For longer term storage, freeze working aliquots at -70°C.
Applications and Suggested Working Dilutions
This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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