A-20 Whole Cell Lysate

A-20 Whole Cell Lysate

Product No.: A232

[product_table name="All Top" skus="A232"]

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Product Type
Cell Line Lysate

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Details

Cell Line
A-20 (B Lymphocyte; Reticulum Cell Sarcoma)
Tissue Type
Tumor
Growth Media
RPMI 1640 Medium with 2 mM L-Glutamine & 10% FBS (Fetal Bovine Serum)
Preparation
A-20 lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecyl sulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay.
Host
Mouse
Concentration
2.0 mg/ml
Formulation
The lysate was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecyl sulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.
Expiration Date
One year from date of receipt
Storage
This lysate should be aliquoted in working amounts and may be stored for up to one month at -20°C <b>in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.</b> For longer term storage, freeze working aliquots at -70°C.
Applications and Suggested Working Dilutions
This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.